一株α-淀粉酶产生菌株的分离鉴定及其产酶条件优化  

Isolation and identification of anα-amylase producing strain and optimization of its enzymatic production conditions

作　　者：王圣心 王力 张建国 许琪 寻立之 李晓华[1] WANG Shengxin;WANG Li;ZHANG Jianguo;XU Qi;XUN Lizhi;LI Xiaohua(College of Life Sciences&Hubei Provincial Engineering and Technology Research Center for Resources and Utilization of Microbiology,South-Central Minzu University,Wuhan 430074,China;Jiangsu Shenli Ecological Agricultural Science and Technology Co.,Ltd.,Yixing 214221,China)

机构地区：[1]中南民族大学生命科学学院&微生物资源与利用湖北省工程技术研究中心,武汉430074 [2]江苏神力生态农业科技有限公司,宜兴214221

出　　处：《中南民族大学学报（自然科学版）》2023年第1期58-64,共7页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家自然科学基金资助项目(31070087);中央高校基本科研业务费专项基金资助项目(CZY19018);企业委托资助项目(HZY20042,HZY21015)。

摘　　要：从湖南省长沙县养殖场牛胃秸秆发酵物中筛选获得了一株高产α-淀粉酶的菌株,命名为SCUEC8.通过对其形态学观察和生理生化特性,结合16S rDNA序列对比分析,初步鉴定该菌为枯草芽孢杆菌(Bacillus subtilis).探讨了不同碳源、氮源、培养条件等因素对枯草芽孢杆菌SCUEC8菌株产酶的影响,结果表明:在培养时间为16 h、pH为6.0、培养温度为37℃、碳源为20.0 g·L^(-1)的麦芽糖、氮源为15.0 g·L^(-1)的胰蛋白胨、金属离子为1.0 g·L^(-1)的Mn^(2+)时,枯草芽孢杆菌SCUEC8菌株生长量和α-淀粉酶的活性较高.A strain with high production ofα-amylase was selected from the straw fermentation of cattle stomach in Changsha County,Hunan Province,and was named SCUEC8.Based on the morphological observation,physiological and biochemical characteristics,and 16S rDNA sequence comparison analysis,the bacterium was preliminarily identified as Bacillus subtilis.Meanwhile,the effects of different factors such as carbon sources,nitrogen sources,and culture conditions on the enzymatic production of Bacillus subtilis SCUEC8 strain were investigated.The results demonstrated that the optimal culture conditions for the better growth of Bacillus subtilis SCUEC8 strain and higherα-amylase activity were as follows:16 h of culture time,pH 6.0,37℃of culture temperature,20.0 g·L^(-1) maltose as carbon source,15.0 g·L^(-1) tryptone as nitrogen source,and 1.0 g·L^(-1) Mn^(2+)as mental ion.

关 键 词：Α-淀粉酶 枯草芽孢杆菌SCUEC8菌株 产酶条件优化 

分 类 号：Q93[生物学—微生物学]