Kinetic modeling of gamma-aminobutyric acid production by Lactobacillus brevis based on pH-dependent model and rolling correction  

作　　者：Min Wu Juanjuan Ding Zhaofeng Zhang Shengping You Wei Qi Rongxin Su Zhimin He 

机构地区：[1]Chemical Engineering Research Center,School of Chemical Engineering and Technology,Tianjin University,Tianjin 300350,China [2]State Key Laboratory of Chemical Engineering,Tianjin University,Tianjin 300350,China [3]Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),Tianjin 300072,China [4]Tianjin Key Laboratory of Membrane Science and Desalination Technology,Tianjin University,Tianjin 300072,China

出　　处：《Chinese Journal of Chemical Engineering》2022年第10期352-360,共9页中国化学工程学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(21621004,22078239);the Beijing-Tianjin-Hebei Basic Research Cooperation Project(B2021210008);Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(TSBICIP-KJGG-004);the Tianjin Development Program for Innovation and Entrepreneurship(2018)。

摘　　要：Gamma-aminobutyric acid(GABA)is a natural non-protein functio nal amino acid,which has potential for fermentation industrial production by Lactobacillus brevis.This work investigated the batch fermentation process and developed a kinetic model based on substrate restrictive model established by experimental data from L25(5~6)orthogonal experiments.In this study,the OD600 value of fermentation broth was fixed to constant after reaching its maximum because the microorganism death showed no effect on the enzyme activity of glutamate decarboxylase(GAD).As pH is one of the key parameters in fermentation process,a pH-dependent kinetic model based on radial basis function was developed to enhance the practicality of the model.Furthermore,as to decrease the deviations between the simulated curves and the experimental data,the rolling correction strategy with OD600 values that was measured in real-time was introduced into this work to modify the model.Finally,the accu racy of the rolling corrected and pH-dependent model was validated by good fitness between the simulated curves and data of the initial batch fermentation(pH 5.2).As a result,this pH-dependent kinetic model revealed that the optimal pH for biomass growth is 5.6-5.7 and for GABA production is about 5,respectively.Therefore,the developed model is practical and convenient for the instruction of GABA fermentation production,and it has instructive significance for the industrial scale.

关 键 词：Kinetic modeling FERMENTATION Gamma-aminobutyric Lactobacillus brevis pH control Rolling correction 

分 类 号：TQ922[轻工技术与工程—发酵工程]