大麦种质资源抗叶斑病评价  

Evaluation of Resistance to Spot Blotch in Core Germplasm Resources

作　　者：孙逸凡 黄志磊 李葆春[1,3] 姚立蓉 汪军成[1,2] 司二静[1,2] 杨轲[1,2] 孟亚雄[1,2] 马小乐[1,2] 王化俊[1,2] SUN Yifan;HUANG Zhilei;LI Baochun;YAO Lirong;WANG Juncheng;SI Erjing;YANG Ke;MENG Yaxiong;MA Xiaole;WANG Huajun(State Key Laboratory of Aridland Crop Science,Gansu Provincial Key Laboratory of Crop Improvement&Germplasm Enhancement,Gansu Agricultural University,Lanzhou 730070,China;College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China;College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学省部共建干旱生境作物学国家重点实验室,甘肃省作物遗传改良与种质创新重点实验室,兰州730070 [2]甘肃农业大学农学院,兰州730070 [3]甘肃农业大学生命科学技术学院,兰州730070

出　　处：《中国农业科技导报》2022年第11期43-54,共12页Journal of Agricultural Science and Technology

基　　金：财政部和农业农村部国家现代农业产业技术体系建设项目(CARS-05-04B-2);甘肃省教育厅产业支撑计划项目(2021CYZC-12);国家自然科学基金项目(32001514,31960072);973计划前期研究专项(2014CB160313);甘肃省自然基金项目(20JR10RA507);甘肃农业大学伏羲青年英才项目(GANfx-03Y06,GAUfx-04Y011)。

摘　　要：为明确不同大麦种质对叶斑病的抗性差异,筛选抗性种质材料和挖掘相关的遗传位点,在三叶期对200份大麦种质接种叶斑病强致病株Z14484,培养9 d后统计各材料发病情况,明确其抗病等级,进行抗性鉴定与评价。结合Illumina 9K SNP芯片,通过Structure和PLINK软件进行群体和显著位点分析,鉴定与性状显著关联的SNP位点。结果表明,供试大麦材料抗性差异显著,在ΔK=3水平上群体遗传结构可以分为3个亚群,分别包含100、67、33份大麦材料;全基因组关联分析(genome-wide association study,GWAS)共检测到12个与大麦叶斑病抗性相关的位点,其中位于3H染色体上的11_11436和SCRI_RS_201075与大麦叶斑病抗性关联最大。研究结果为大麦叶斑病抗病基因定位与抗病育种提供参考依据。In order to clarify the resistance differences of different barley germplasms to leaf spot,the resistant germplasm materials were screened and related genetic loci were mined. In this study,200 barley germplasms were inoculated with Z14484,a highly pathogenic strain of barley leaf spot,at three-leaf stage. After cultured for 9 d,the incidence of each material was counted,the disease resistance grade was clarified,and resistance identification and evaluation were carried out. Combined with Illumina 9K SNP chip,the population and significant loci were analyzed by Structure software and PLINK software to identify SNP loci significantly associated with traits. The results showed that there were significant differences in the resistance among the tested barley materials,and the population genetic structure could be divided into 3 subgroups at ΔK=3 level,including 100,67 and 33 barley materials,respectively.11_11436 and SCRI_RS_201075 located on chromosome 3H were most associated with resistance to barley leaf spot by genome-wide association study. This study provided a reference for barley leaf spot disease resistance gene mapping and disease resistance breeding.

关 键 词：大麦叶斑病 抗性鉴定 SNP 全基因组关联分析 

分 类 号：S512.3[农业科学—作物学]