miR-18b-5p在肝癌早期筛查中的临床应用价值研究  

Clinical value of mir-18b-5p in early screening of liver cancer

作　　者：钟小青[1] 江文力[1] 潘小平[1] 胡海春 ZHONG Xiao-qing;JIANG Wen-li;PAN Xiao-ping(Laboratory Department of Guangzhou Huadu District People's Hospital,Guangzhou 510800,China.)

机构地区：[1]广州市花都区人民医院检验科,广州510800

出　　处：《中国处方药》2022年第12期170-172,共3页Journal of China Prescription Drug

基　　金：广州市花都区科技和信息化局2021年医疗卫生计划项目(21-HDWS-032)。

摘　　要：目的 观察miR-18b-5p在肝癌早期筛查中的临床应用价值。方法 随机选择2021年1月~2021年12月确诊的肝癌患者90例,乙型肝炎患者90例,丙型肝炎50例,健康体检者90例作为研究对象。所有纳入研究患者均给予miR-18b-5p水平测定。冻存于-80℃的血液样本提取总RNA,qRT-PCR法检测miR-18b-5p表达,计算PCR的扩增的Ct,比较四组纳入研究人员的miR-18b-5p水平,肝癌组患者不同性别、年龄、分期、淋巴结转移、有无腹水患者的miR-18b-5p水平。通过ROC曲线评价PCR检测miR-18b-5p的在肝癌中诊断效能。结果 肝脏组患者miR-18b-5p水平高于乙型肝炎、丙型肝炎和对照组,乙型肝炎和丙型肝炎患者miR-18b-5p水平高于对照组,差异均具有统计学意义(P <0.05),但乙型肝炎和丙型肝炎组患者miR-18b-5p水平无明显差异(P> 0.05)。肝癌组患者根据不同病理特征分组,肿瘤大小超过5 cm患者的miR-18b-5p水平高于肿瘤大小<5 cm,TNM分期为Ⅲ期患者的miR-18b-5p水平高于Ⅰ+Ⅱ期水平,差异均具有统计学意义(P <0.05),但是不同性别、年龄以及是否合并肝硬化患者miR-18b-5p水平差异无统计学意义(P> 0.05)。通过ROC曲线评价PCR检测miR-18b-5p的在肝癌中诊断效能,其灵敏度为95.77%,特异度为87.23%。结论 miR-18b-5p对于肝癌的诊断、鉴别诊断具有重要的应用价值,尤其是对于肝癌的早期筛查意义重大。Objective To observe the clinical value of miR-18b-5p in early screening of liver cancer. Methods 90 patients with liver cancer, 90 patients with hepatitis B, 50 patients with hepatitis C and 90 healthy people diagnosed in our hospital from January 2021 to December 2021 were randomly selected as the research objects. All patients included in the study were given miR-18b-5p level determination. Total RNA was extracted from blood samples frozen at-80℃, the expression of miR-18b-5p was detected by qRT-PCR, and the amplified Ct of PCR was calculated. The levels of miR-18b-5p of researchers included in the four groups were compared, and the levels of miR-18b-5p of patients with liver cancer in different gender, age, stage, lymph node metastasis and ascites were compared. The diagnostic efficacy of PCR detection of miR-18b-5p in hepatocellular carcinoma was evaluated by ROC curve. Results The level of miR-18b-5p in liver group was higher than that in hepatitis B, hepatitis C and the control group, and the level of miR-18b-5p in hepatitis B and hepatitis C was higher than that in the control group(P < 0.05), but there was no significant difference in the level of miR-18b-5p between hepatitis B and hepatitis C group(P > 0.05). According to different pathological characteristics, the level of miR-18b-5p in patients with tumor size more than 5 cm was higher than that in patients with tumor size less than 5 cm, and the level of miR-18b-5p in patients with TNM stage III was higher than that in patients with stage I + II(P< 0.05), but there was no significant difference in the level of miR-18b-5p in patients with different gender, age and whether complicated with liver cirrhosis(P > 0.05). The diagnostic efficacy of PCR for miR-18b-5p in hepatocellular carcinoma was evaluated by ROC curve. The sensitivity was 95.77% and the specificity was 87.23%. Conclusion miR-18b-5p has important application value for the diagnosis and differential diagnosis of liver cancer, especially for the early screening of liver cancer.

关 键 词：miR-18b-5p 肝癌 早期筛查 

分 类 号：R735.7[医药卫生—肿瘤]