芦荟胶抗炎功效研究  

Study on the anti-inflammatory effect of Aloe vera gel

作　　者：李晓敏 张红霞 范莹 袁利文 汤酿 郑锦荣 闫永涛 Xiaomin Li;Hongxia Zhang;Ying Fan;Liwen Yuan;Niang Tang;Jinrong Zheng;Yongtao Yan(Perfect(Guangdong)Commodity Co.,Ltd.,Zhongshan,Guangdong 528400,China;Perfect Life Sciences Research Institute Co.,Ltd.,Zhongshan,Guangdong 528400,China)

机构地区：[1]完美(广东)日用品有限公司,广东中山528400 [2]广东完美生命健康科技研究院有限公司,广东中山528400

出　　处：《日用化学工业（中英文）》2022年第12期1320-1325,共6页China Surfactant Detergent & Cosmetics

摘　　要：初步探讨芦荟胶的抗炎功效和作用机制。使用佛波酯(TPA)在雄性KM小鼠耳朵部位进行炎症造模,并制成切片进行HE染色;应用硫酸铜诱导斑马鱼胚胎侧线区域神经丘细胞损伤,研究了中性粒细胞聚集情况;采用LPS诱导RAW264.7细胞炎症模型考察芦荟胶对炎症因子分泌的抑制作用;分别使用微量肉汤稀释法和悬液定量法研究了芦荟胶对金黄色葡萄球菌、痤疮丙酸杆菌的最小抑菌浓度(MIC)和抑菌率。结果显示,芦荟胶在1.5 g/mL时可抑制小鼠耳朵肿胀(P<0.05);芦荟胶在0.7和2.50 mg/mL时对斑马鱼胚胎中性粒细胞聚集有显著抑制(P<0.01);芦荟胶在0.75,1.50,3.00 mg/mL时可显著抑制RAW264.7细胞分泌TNF-α、IL-6(P<0.01);芦荟胶对金黄色葡萄球菌和痤疮丙酸杆菌的MIC分别是12.5和3.125 mg/mL,对金黄色葡萄球菌、痤疮丙酸杆菌的抑菌率随着芦荟胶的作用时间延长和浓度增加而升高。芦荟胶具有抗炎功效,且该功能可能通过减少中性粒细胞聚集、抑制炎症因子TNF-α和IL-6产生的抗炎通路,以及抑制金黄色葡萄球菌和痤疮丙酸杆菌的抑菌功能共同实现。This study is to dissect the effect and mechanism of Aloe vera gel on anti-inflammation.The ear inflammation model of male Kunming(KM)mice was induced by phorbol ester(TPA).The accumulation of neutrophils in the lateral line region of zebrafish embryos was tested using copper sulfate.The inhibitory effect of Aloe vera gel on the secretion of cytokines in RAW264.7 cells,including TNF-αand IL-6,was studied in lipopolysaccharide(LPS)-induced inflammation model.The minimum inhibitory concentration(MIC)and inhibitory rate of aloe gel against Staphylococcus aureus and Propionibacterium acnes were also studied via broth microdilution method and suspension quantification method,respectively.The model of ear inflammation is successfully established in male KM mice,and Aloe vera gel inhibits the ear swelling of mice at 1.5 g/mL(P<0.05).Aloe vera gel significantly inhibits neutrophil aggregation in zebrafish embryos at 0.7 and 2.50 mg/mL(P<0.01).Aloe vera gel significantly inhibits the secretion of TNF-αand IL-6 in RAW264.7 cells at 0.75,1.50 and 3.00 mg/mL(P<0.01).The MIC of aloe gel against Staphylococcus aureus and Propionibacterium acnes are 12.5 and 3.125 mg/mL,respectively.The inhibitory rate of aloe gel against Staphylococcus aureus and Propionibacterium acnes increases with the prolongation of the action time and concentration of Aloe vera gel.Aloe vera gel achieves anti-Inflammatory effect possibly through anti-inflammatory pathway of reducing neutrophil aggregation and inhibiting the production of inflammatory factors TNF-αand IL-6,as well as directly inhibiting Staphylococcus aureus and Propionibacterium acnes.

关 键 词：芦荟胶 抗炎 抑菌 RAW264.7细胞 

分 类 号：TQ658[化学工程—精细化工]