Foxp3/SMARCE1信号轴促进宫颈癌细胞侵袭和迁移  

Foxp3/SMARCE1 signal axis promotes invasion and migration of cervical cancer cells

作　　者：龙嘉莉 刘曙光 马红梅 曾超[1] LONG Jia-li;LIU Shu-guang;MA Hong-mei;ZENG Chao(Department of Pathology,the Eighth Affiliated Hospital,Sun Yat-sen University,Shenzhen 518033,Guangdong,China)

机构地区：[1]中山大学附属第八医院病理科,广东深圳518033

出　　处：《川北医学院学报》2022年第12期1515-1518,共4页Journal of North Sichuan Medical College

基　　金：国家自然科学基金青年项目(81302245);深圳市福田区卫生公益科研项目(FTWS2019015、FTWS2018045)。

摘　　要：目的:探讨叉头样转录因子3(Foxp3)是否通过SMARCE1促进宫颈癌Hela细胞的侵袭和迁移。方法:基因芯片筛选下调Foxp3后下游差异表达基因。宫颈癌Hela细胞转染Foxp3 shRNA干扰后,Western blot和细胞免疫荧光实验检测SMARCE1的表达。Transwell迁移及侵袭实验观察Hela细胞共转染Foxp3 shRNA和SMARCE1质粒后迁移和侵袭情况。结果:基因芯片结果显示SMARCE1是Foxp3的下游基因;Western blot和细胞免疫荧光实验结果表明,Foxp3增强Hela细胞中SMARCE1蛋白的表达;Transwell迁移及侵袭实验证实Foxp3增强SMARCE1促进Hela细胞的迁移和侵袭。结论:Foxp3通过调控SMARCE1促进宫颈癌Hela细胞的侵袭和迁移,Foxp3/SMARCE1信号轴在宫颈癌的进展中可能起着重要作用。Objective:To investigate whether Foxp3 can promote invasion and migration of cervical cancer Hela cells by SMARCE1.Methods:The downstream differential expression genes of Foxp3 down-regulated were screened by gene chip.After Hela cells were transfected with Foxp3 shRNA interference,the expression of SMARCE1 was detected by Western blot and immunofluorescence assay.Transwell migration and invasion assay was used to observe the migration and invasion of Hela cells after co-transfection with Foxp3 shRNA and SMARCE1 plasmids.Results:Microarray results showed that SMARCE1 was the downstream gene of Foxp3.Western blot and immunofluorescence assay showed that Foxp3 enhanced the expression of SMARCE1 protein in Hela cells.Transwell migration and invasion assay confirmed that Foxp3 enhanced SMARCE1 and promoted migration and invasion of Hela cells.Conclusion:Foxp3 promotes invasion and migration of cervical cancer Hela cells by regulating SMARCE1,and Foxp3/SMARCE1 signaling axis may play an important role in the progression of cervical cancer.

关 键 词：宫颈癌 叉头样转录因子3 SMARCE1 侵袭 迁移 

分 类 号：R737.33[医药卫生—肿瘤]