在大肠杆菌体内有效地表达以组蛋白为骨架的融合蛋白HNHG  

Expression of Histone-based Fusion Protein HNHG inE.coli

作　　者：戴菲寒[1] 陈燕[2] 龚毅[2] 杨胜利[2] 田培坤[1] 顾健人[1] 

机构地区：[1]上海市肿瘤研究所癌基因及相关基因国家重点实验室,上海200032 [2]中国科学院上海生物工程研究中心,上海200233

出　　处：《生物化学与生物物理学报》2002年第6期800-803,共4页

基　　金：国家高技术研究发展计划资助项目 (863计划 ) (No .Z20-01 -01) ;专利申请号 :CN0 2 13 6162 .~~

摘　　要：研究了在大肠杆菌体内表达了以组蛋白C 末端为骨架的融合蛋白HNHG[H(HA2 0 )N(NLS)H(组蛋白H10 的C末端 97个氨基酸 )G(GE7) ],并发现质粒DNA与其形成复合体后 ,在电镜下出现与体内染色体相同的凝聚、螺旋现象。由于HNHG的这种有效地结合质粒、并使之凝聚的特性 ,有望使之成为新兴的能将外源基因导入体内的载体系统。Histone H1 contributes to condense nucleosome into super-structure during the transformation of chromatin into chromosome. It is shown in this report that the fusion protein HNHG with the core of C- terminus of histone H10 expressed in BL21 (DE3) could also condense the plasmid DNA, just as histones did in nucleus. Under electron microscope, plasmid DNA condensed and supercoiled after the addition of HNHG, in contrast to plasmid DNA control. This specific ability of the fusion protein HNHG of binding and condensing plasmids could be utilized to construct novel exogenous gene delivery systems. HNHG would be a promising candidate for gene delivery.

关 键 词：大肠杆菌 表达 组蛋白 融合蛋白HNHG 基因工程 基因导入载体 

分 类 号：Q786[生物学—分子生物学]