消岩汤含药血清培养的乳腺癌MCF-7细胞增殖、迁移、侵袭能力变化及其机制  

Effects of Xiaoyan decoction-containing serum on proliferation,migration,and invasion of MCF-7 cells

作　　者：吕艳[1] 耿强[1] 韩燕燕[1] 郑旭[1] 李翀[1] 兰福 赵杰[1] LYU Yan;GENG Qiang;HAN Yanyan;ZHENG Xu;LI Chong;LAN Fu;ZHAO Jie(Department of Pathology,The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300381,China)

机构地区：[1]天津中医药大学第一附属医院病理科国家中医针灸临床医学研究中心,天津300381

出　　处：《山东医药》2022年第35期14-17,39,共5页Shandong Medical Journal

基　　金：天津市教委科研计划项目(2017KJ160)。

摘　　要：目的 观察消岩汤含药血清对乳腺癌MCF-7细胞增殖、迁移和侵袭能力的影响,并探讨其机制是否与调控circRIP2和转化生长因子β(TGF-β)有关。方法 取对数生长期的MCF-7细胞,分为消岩汤含药血清4组、消岩汤含药血清2组、消岩汤含药血清1组、普通牛血清组、空白鼠血清组,分别加入浓度比为4∶2∶1的消岩汤含药血清、普通10%小牛血清及空白鼠血清100μL,孵育24 h。采用CCK-8法检测各组培养24、48 h的细胞增殖能力(以吸光度值表示),细胞划痕实验检测培养24、48、72 h的细胞迁移能力(以细胞迁移率表示),Transwell小室实验检测细胞侵袭能力(以穿膜细胞数表示),qRT-PCR法检测细胞circRIP2及RIP2、TGF-βmRNA表达,Western blotting法检测细胞丝氨酸苏氨酸激酶2(RIPK2)、TGF-β蛋白表达。结果 培养24 h,消岩汤含药血清4、2组细胞增殖能力均低于普通牛血清组(P均<0.05);培养48 h,消岩汤含药血清4、2、1组细胞增殖能力均低于普通牛血清组,消岩汤含药血清4组细胞增殖能力均低于空白鼠血清组(P均<0.05)。与普通牛血清组、空白鼠血清组同时间点比较,消岩汤含药血清4、2、1组24 h细胞迁移率及穿膜细胞数均降低,消岩汤含药血清4、2组48、72 h细胞迁移率均降低(P均<0.05)。消岩汤含药血清4组细胞circRIP2、TGF-βmRNA相对表达量均低于普通牛血清组、空白鼠血清组,RIP2mRNA相对表达量均低于普通牛血清组(P均<0.05)。消岩汤含药血清4组RIPK2蛋白相对表达量低于空白鼠血清组、消岩汤含药血清1组,TGF-β蛋白相对表达量低于普通牛血清组(P均<0.05)。结论 高浓度消岩汤含药血清可通过降低circRIP2、TGF-β表达而参与抑制乳腺癌MCF-7细胞的增殖、迁移和侵袭。Objective To observe the effects of Xiaoyan decoction-containing serum on the proliferation,migration and invasion of MCF-7 cells,and to explore whether the mechanism is related to the regulation of circ RIP2 and transforming growth factor-β(TGF-β)or not.Methods MCF-7 cells in the logarithmic growth phase were divided into five groups:Xiaoyan decoction-containing serum group 4,Xiaoyan decoction-containing serum group 2,Xiaoyan decoctioncontaining serum group 1,bovine serum group,and mouse serum group.Xiaoyan decoction-containing serum(100μL)at a concentration ratio of 4∶2∶1,10%calf serum and mouse serum were added to the cells,then the cells were incubated for24 h.CCK-8 was used to detect the proliferation of MCF-7 at 24 and 48 h(OD value).The migration of MCF-7 at 24,48and 72 h(cell migration rate)was detected by Scratch test.Transwell test was applied to detect the invasion of cells at 24,48 and 72 h(number of trans-membrane cells).The m RNA level of circ RIP2,RIP2,and TGF-βwere detected by q RT-PCR.The protein levels of RIPK2 and TGF-βwere detected by Western blotting.Results After 24 h of culture,the cell proliferation abilities of Xiaoyan decoction-containing serum groups 4 and 2 were lower than that of the bovine serum group(both P<0.05).After 48 h of culture,the cell proliferation abilities of Xiaoyan decoction-containing serum groups4,2,and 1 were lower than that of the common bovine serum group(all P<0.05).The cell proliferation of Xiaoyan decoction-containing serum group 4 was lower than that of mouse serum group(P<0.05).Compared with the bovine serum group and mouse serum group at the same time point,the cell migration rate and the number of transmembrane cells decreased in the Xiaoyan decoction-containing serum groups 4,2 and 1 at 24 h(all P<0.05),and the cell migration rate decreased in the Xiaoyan decoction-containing serum groups 4 and 2 at 48 and 72 h(all P<0.05).The m RNA levels of circ RIP2 and TGF-βin Xiaoyan decoction-containing serum group 4 were lower than those in the bovine serum

关 键 词：消岩汤 乳腺癌 细胞增殖 细胞迁移 细胞侵袭 环状核糖核酸 circRIP2 转化生长因子Β2 

分 类 号：R737.9[医药卫生—肿瘤]