茶网蝽线粒体基因组全序列测定及系统发育分析  

The Complete Mitochondrial Genome Sequence and Phylogenetic Analysis of the Stephanitis chinensis

作　　者：杨金宏 谢满超 文欣茹 陈蕊茹 孔卫青[2] YANG Jinhong;XIE Manchao;WEN Xinru;CHEN Ruiru;KONG Weiqing(School of Modern Agriculture&Biotechnology,Shaanxi Tea Key Lab Co-sponsored by Province and Municipality,Ankang University,Ankang 725000,China;Shaanxi Key Laboratory of Sericulture,Ankang University,Ankang 725000,China)

机构地区：[1]安康学院现代农业与生物科技学院/陕西省茶叶省市共建重点实验室,陕西安康725000 [2]安康学院陕西省蚕桑重点实验室,陕西安康725000

出　　处：《茶叶科学》2022年第6期839-850,共12页Journal of Tea Science

基　　金：陕西省重点研发计划(2018ZDXM-NY-057);安康市科技发展计划(AK2021-NY-2);安康学院科研项目(2017AYXNZX11)。

摘　　要：为明确茶网蝽(Stephanitis chinensis)线粒体基因组序列特征,探究其系统发育地位。利用Illumina和Sanger测序对陕西省安康市茶网蝽线粒体基因组进行测定。结果显示,茶网蝽线粒体基因组全长18085 bp,包含37个基因(13个蛋白质编码基因,22个tRNA,2个rRNA)和1个3678 bp的控制区,基因排列与昆虫线粒体祖先基因顺序(Ancestralgeneorder)相同。基因组AT含量为78.10%。13个蛋白质编码基因中,6个以ATG起始,7个以ATT或ATA起始,10个以TAA或TAG为终止密码子,cox2、atp6和cox3以T终止,蛋白质编码基因使用频率最高的密码子是UUA、AUU、UUU和AUA,使用频率最高的氨基酸为亮氨酸(Leu)、异亮氨酸(Ile)、苯丙氨酸(Phe)和丝氨酸(Ser)。22个tRNA基因存在GU、UU、GA和AA错配23处,trnS1(GCU)缺少DHU臂,其他tRNA均能形成典型三叶草结构。控制区包含位于前端的3种非串联重复、4个(TTAG)_(n)和1个位于后端的串联重复序列,含有多个茎环结构。系统发育分析结果表明,茶网蝽与直脊冠网蝽(Stephanitis mendica)的亲缘关系最近,所有网蝽科聚为一簇,位于发育树的根部。In order to obtain the characteristics of the mitochondrial genome(mitogenome)and explore phylogenetic relationship,the mitogenome sequence of Stephanitis chinensis collected from Ankang city,Shaanxi Province was determined by Illumina and Sanger sequencing methods.The result shows that the mitogenome of S.chinensis is 18085 bp in length,including 37 coding genes[13 protein-coding genes(PCGs),22 tRNAs,and 2 rRNAs],and one control region of 3678 bp.The gene organization of S.chinensis is the same as the ancestral gene order of insect mitogenome.The AT content of whole mitogenome is 78.10%.Among the 13 PCGs,six start with the typical ATG,seven start with ATT or ATA,and ten end with the typical TAA or TAG.Only cox2,atp6 and cox3 terminate with T.The highly occurred codons in the PCGs are UUA,AUU,UUU and AUA,and the top 4 amino acids are Leu,Ile,Phe and Ser.There are 23mismatches of GU,UU,GA and AA in 22 tRNAs.All the tRNAs could form the common clover-leaf secondary structures,except trnS1(GCU),which lacks the DHU arm.The control region contains 3 type of non-tandem repeats and 4(TTAG)_(n) in the front-end region(FER)and one tandem repeat in the back-end region(BER),and several stem-loop structures.Phylogenetic analysis shows that Stephanitis mendica has the closest relationship with S.chinensis.All the insects from Tingidae were clustered together,and placed at the base part of the phylogenetic tree.

关 键 词：茶网蝽 线粒体基因组 序列分析 系统发育 

分 类 号：S571.1[农业科学—茶叶生产加工] S435.711[农业科学—作物学]