不同糖耐量乳母产后42天母乳微生物群落差异  

Breast milk microbiota of mothers with different glucose tolerance at 42 days postpartum

作　　者：朱鸿妮 林松 叶慧 陈洁 黄芳 陈婕 郭福川 Zhu Hongni;Lin Song;Ye Hui;Chen Jie;Huang Fang;Chen Jie;Guo Fuchuan(Department of Nutrition and Food Safety,School of Public Health,Fujian Medical University,Fuzhou 350100,China;Fuqing Maternity and Child Health Care Hospital,Fuzhou 350301,China)

机构地区：[1]福建医科大学公共卫生学院营养与食品安全学系,福州350100 [2]福清市妇幼保健院,福州350301

出　　处：《卫生研究》2022年第6期988-995,共8页Journal of Hygiene Research

基　　金：福建医科大学高层次人才科研启动经费(No.XRCZX2017002)。

摘　　要：目的比较妊娠期糖尿病(gestational diabetes mellitus,GDM)与对照乳母产后42天母乳微生物多样性差异,探讨GDM对母乳微生物组成和结构的影响。方法于2019年5月至2020年9月,在福建省福清市妇幼保健院选择足月分娩的25例孕期血糖正常(对照组)及21例诊断为GDM的母婴(GDM组)为研究对象,问卷调查了解乳母的基本信息和膳食摄入情况,使用无菌电动母乳泵收集母乳。采用16S rDNA高通量测序技术对V3-V4可变区进行PCR扩增,利用Miseq PE300平台进行测序,对测序后的结果进行生物信息学分析。结果蔬菜类摄入量[M(P25,P75)]GDM组高于对照组[222.7(190.6,333.1)g/d比176.4(49.5,247.0)g/d,P<0.05]。两组母乳微生物的Alpha多样性比较差异无统计学意义(P>0.05)。而Beta多样性分析显示,两组母乳的微生物构成差异有统计学意义(P<0.01)。菌群差异分析显示,在门水平上与对照组相比,GDM组的拟杆菌门(Bacteroidetes)和蓝细菌门(Cyanobacteria)的相对丰度显著降低[(3.41±2.59)%比(1.23±0.82)%,(1.08±3.02)%比(0.10±0.11)%,P<0.05];在属水平上与对照组相比,GDM组母乳罗尔斯通菌属(Ralstonia)、红球菌属(Rhodococcus)、Burkholderia-Caballeronia-Paraburkholderia菌属、不动杆菌属(Acinetobacter)、Fluviicola菌属显著降低[(38.93±28.85)%比(26.70±28.37)%,(9.23±6.87)%比(4.88±6.03)%,(7.66±4.80)%比(2.77±1.33)%,(6.18±11.90)%比(2.76±6.10)%,(1.21±1.31)%比(0.33±0.62)%,P<0.05],黄杆菌科中的未分类细菌属(unclassified_f__Xanthobacteraceae)显著增加[(0.85±3.15)%比(23.64±23.63)%,P<0.05]。结论不同糖耐量乳母产后42天母乳微生物群落结构存在差异,罗尔斯通菌属(Ralstonia)、红球菌属(Rhodococcus)、Burkholderia-Caballeronia-Paraburkholderia菌属、不动杆菌属(Acinetobacter)、Fluviicola菌属在GDM母亲的母乳中相对丰度显著低于糖耐量正常母亲的母乳。OBJECTIVE To compare the differences in microbial community between lactating mothers with gestational diabetes mellitus(GDM)and normal glucose tolerance lactating mothers(control)at 42 days postpartum,and to explore the effect of GDM on the microbial composition and structure of breast milk.METHODS A total of 21 mothers with GDM and 25 healthy mothers in Fuqing Maternity and Child Health Care Hospital at 42 days postpartum from May 2019 to September 2020 were enrolled.A questionnaire was used to collect the basic information and dietary intakes of mothers.The mother’s milk was collected by using a sterile electric breast pump.Breast milk microbiota profiles were assessed by 16 S rDNA gene amplicon based sequencing of the V3-V4 region and the sequencing platform was Illumina Miseq PE3000,bioinformatics analysis was performed on the sequencing result.RESULTS Compared with the control group,the mothers in the GDM group consumed more vegetables(222.7(190.6,333.1)g/d vs.176.4(49.5,247.0)g/d,P=0.042).There was no significant difference in Alpha diversity between the two groups(P>0.05).Beta diversity analysis showed that there were significant differences in the microbial composition between the two groups(P<0.01).Breast milk microbiota species difference analysis showed that there were differences in several species between GDM group and NGT group at the levels from phylum to genus.Compared with control group,the relative abundance of Bacteroidota and Cyanobacteria in GDM group decreased significantly[(3.41±2.59)%vs.(1.23±0.82)%,(1.08±3.02)%vs.(0.10±0.11)%,P<0.05].In the GDM group,Ralstonia,Rhodococcus,Burkholderia-Caballeronia-Paraburkholderia,Acinetobacter and Fluviicola were decreased((38.93±28.85)%vs.(26.70±28.37)%,(9.23±6.87)%vs.(4.88±6.03)%,(7.66±4.80)%vs.(2.77±1.33)%,(6.18±11.90)%vs.(2.76±6.10)%,(1.21±1.31)%vs.(0.33±0.62)%,P<0.05).The unclassified_f__xanthobacteraceae was increased,and the difference was statistically significant((0.85±3.15)%vs.(23.64±23.63)%,P<0.05).CONCLUSION There are diffe

关 键 词：妊娠期糖尿病 母乳微生物 菌群结构 膳食摄入量 相对丰度 

分 类 号：R173[医药卫生—妇幼卫生保健] R153.1[医药卫生—公共卫生与预防医学] R378