卵巢癌组织中lncRNA NORAD的表达水平及其与临床病理特征和预后的相关性  

Expression level of lncRNA NORAD in ovarian cancer tissues and its correlation with clinicopathological features and prognosis

作　　者：隋磊 纪晓宇 朱晓瑛 杜秀珍 尤师静 姚勤[2] SUI Lei;JI Xiaoyu;ZHU Xiaoying;DU Xiuzhen;YOU Shijing;YAO Qin(Medical College,Qingdao University,Shandong Qingdao 266071,China;Department of Gynecology,the Affiliated Hospital of Qingdao University,Shandong Qingdao 266071,China)

机构地区：[1]青岛大学医学部,山东青岛266071 [2]青岛大学附属医院妇科,山东青岛266071

出　　处：《现代肿瘤医学》2023年第1期152-155,共4页Journal of Modern Oncology

基　　金：山东省自然科学基金(编号:ZR2016HM08);山东省青岛市民生科技计划资助项目(编号:19-6-46-nsh)。

摘　　要：目的:探讨卵巢癌组织中DNA损伤激活的非编码RNA(non-coding RNA activated by DNA damage,NORAD)的表达水平及其与临床病理特征和预后的相关性。方法:选取2019年至2020年于青岛大学附属医院诊治的50例卵巢癌患者和50例正常卵巢患者的组织标本。通过实时荧光定量反转录聚合酶链式反应(qRT-PCR)方法检测以上患者组织中NORAD的相对表达水平,并分析NORAD的表达水平与临床病理特征和预后的关系。使用Kaplan-Meier plotter在线数据库评估NORAD的表达与卵巢癌预后的相关性。结果:NORAD在卵巢癌组织中呈高表达,其表达与FIGO分期、组织类型、腹水以及淋巴结转移相关(P<0.05)。NORAD高表达的卵巢癌患者5年无进展生存期(progression-free survival,PFS)显著低于低表达组。NORAD在卵巢癌组织中的表达水平在FIGO分期、病理分级、TP53状态以及化疗方面对患者的预后具有评估价值。结论:卵巢癌组织中NORAD表达水平明显升高,且其水平与卵巢癌临床病理特征及预后相关。Objective:To investigate the correlation between the expression level of non-coding RNA activated by DNA damage(NORAD)in ovarian cancer tissues and clinicopathological features and prognosis.Methods:Tissue specimens from 50 patients with ovarian cancer and 50 patients with normal ovaries who were diagnosed and treated in the Affiliated Hospital of Qingdao University from 2019 to 2020 were selected.The relative expression level of NORAD in the tissues of the above patients was detected by real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR)method,and the relationship between the expression level of NORAD and clinicopathological characteristics and prognostic value were analyzed.The association of NORAD expression with ovarian cancer prognosis was assessed using the Kaplan-Meier plotter online database.Results:NORAD was highly expressed in ovarian cancer tissues.The high expression of NORAD in ovarian cancer tissues was correlated with FIGO stage,tumor histotype,ascites and lymph nodes metastasis(P<0.05).The 5-year progression-free survival(PFS)of ovarian cancer patients with high NORAD expression was significantly lower than that of the low expression group.The expression level of NORAD in ovarian cancer tissue had an evaluation value for the prognosis of patients in FIGO stage,pathological grade,TP53 status and chemotherapy treatment.Conclusion:The expression level of NORAD in ovarian cancer tissue is significantly increased,and its level is related to the clinicopathological characteristics and prognosis of ovarian cancer.

关 键 词：卵巢癌 NORAD 预后 Kaplan-Meier plotter数据库 

分 类 号：R737.31[医药卫生—肿瘤]