Comprehensive assessment of growth factors,inflammatory mediators, and cytokines in vitreous from patients with proliferative diabetic retinopathy  

作　　者：Qiao-Yun Gong Guang-Yi Hu Su-Qin Yu Tian-Wei Qian Xun Xu 

机构地区：[1]Department of Ophthalmology,Shanghai General Hospital,Shanghai Jiao Tong University,Shanghai 200080,China [2]National Clinical Research Center for Eye Diseases,Shanghai 200080,China [3]Shanghai Key Laboratoryof Ocular Fundus Diseases,Shanghai 200080,China [4]Shanghai Engineering Center for Visual Science and Photomedicine,Shanghai 200080,China [5]Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Disease,Shanghai 200080,China

出　　处：《International Journal of Ophthalmology(English edition)》2022年第11期1736-1742,共7页国际眼科杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China (No.82101132,No.81800878)。

摘　　要：AIM: To assess alterations in growth factors, inflammatory mediators, and cytokines associated with vitreous-retinal diseases in vitreous humor from patients with proliferative diabetic retinopathy(PDR), and to identify potential new treatment targets and strategies.METHODS: Control vitreous samples were collected from patients with macular hole, epiretinal membranes, or rhegmatogenous retinal detachments, and PDR samples from patients with complications of PDR, who required pars plana vitrectomy. Specimens were stored at-80℃ and then investigated by Luminex multi-factor assay. Parametric and nonparametric analyses of demographic characteristics and cytokine expression levels were conducted using SPSS.RESULTS: There were no significant differences in demographic characteristics between patients with and without PDR. Expression levels of growth factors [plateletderived growth factor(PDGF)-AA, glial cell line-derived neurotrophic factor(GDNF), and vascular endothelial growth factor A(VEGFA)], inflammatory mediators [interleukin(IL)-8, IL-11, and tumor necrosis factor-α(TNF-α)] and cytokines [chemokine C-X-C ligand(CXCL)10, interferon-γ(IFN-γ), and granulocyte macrophage-colony stimulating factor(GM-CSF)] were significantly elevated in vitreous humor from patients with PDR compared with those in the control group(all P<0.05). Further, VEGFA levels were lower in patients with PDR treated with anti-VEGF injection than those who were not(P<0.05), and there was no difference between the PDR group treated with anti-VEGF and controls(P>0.05).CONCLUSION: This proof-of-concept study demonstrates the potential for combinational therapeutic strategies to ameliorate diabetic retinopathy progression by targeting growth factors, inflammatory factors, and cytokines, in addition to VEGFA.

关 键 词：VITREOUS proliferative diabetic retinopathy growth factors INFLAMMATION CYTOKINES 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]