芦荟内生细菌LH-01的鉴定及对病原菌抑菌活性的研究  

Isolation of Endophytic Bacteria LH-01 from Aloe barbadensis and Study of the Antibacterial Activity

作　　者：唐凝 赵盛楠 裴冬丽 TANG Ning;ZHAO Shengnan;PEI Dongli(Department of Biology and Food Science,Shangqiu Normal University/Key Laboratory of Plant-Microbe Interactions in Henan Province,Shangqiu 476000,China)

机构地区：[1]商丘师范学院生物与食品学院/植物与微生物互作河南省高校重点实验室,河南商丘476000

出　　处：《东北农业科学》2022年第5期53-56,共4页Journal of Northeast Agricultural Sciences

基　　金：河南省科技攻关项目(222102110003);河南省高等学校重点科研项目(23A210029)。

摘　　要：以库拉索芦荟(Aloe barbadensis)为试验材料,采用平板划线分离法、平板对峙法,分离芦荟内生细菌并研究其对病原菌葡萄座腔菌(Botryosphaeria dothidea)、串珠镰刀菌(Fusarium verticillioides)、禾谷镰刀菌(Fusarium graminearum)、枯萎菌(Fusarium oxysporum)和黄萎菌(Verticillium dahliae)等的抑菌活性。结果表明,筛选出的芦荟内生菌株LH-01对5种病原菌的抑菌率均超过74%。结合生理生化特征、细胞形态特征、菌落形态特征、16S rDNA分子鉴定和系统发育分析,菌株LH-01鉴定为枯草芽孢杆菌类群(Bacillus subtilis subsp.)的Bacillus tequilensis。Strains of endophytic bacteria with antibacterial activity were isolated from Aloe barbadensis by the streak plate method and dual-culture inhibition.Botryosphaeria dothidea,Fusarium verticillioides,Fusarium graminearum,Fusarium oxysporum,Verticillium dahliae were selected for the antifungal experiment.Inhibition rates for the of LH-01 strain to 5 fungi were all more than 74%.LH-01 strain was identified as Bacillus tequilensis through the physiological and biochemical characteristics,cellular and strain morphology,16S rDNA molecular identification and phylogenetic analysis.

关 键 词：库拉索芦荟 内生细菌 枯草芽孢杆菌 抑菌活性 

分 类 号：Q938.1[生物学—微生物学]