小麦不同组织器官叶绿素测定方法的比较研究  

Comparative Study on Determination of Chlorophyll in Different Tissues and Organs of Wheat

作　　者：丁富功 侯泽豪 卢奕霏 宋婧含 孙悦 马东方 刘易科[2] 朱展望[2] 张鹏飞[3] 张迎新 方正武 王书平 DING Fugong;HOU Zehao;LU Yifei;SONG Jinghan;SUN Yue;MA Dongfang;LIU Yike;ZHU Zhanwang;ZHANG Pengfei;ZHANG Yingxin;FANG Zhengwu;WANG Shuping(College of Agriculture,Yangtze University/Hubei Center for Collaborative Innovation of Grain Industry,Jingzhou,434025;Food Crops Institute,Hubei Academy of Agricultural Sciences,Wuhan 430064;Xiangyang Academy of Agricultural Sciences,Xiangyang 441057,China)

机构地区：[1]长江大学农学院/主要粮食作物产业化湖北省协同创新中心,湖北荆州434025 [2]湖北省农业科学院粮食作物研究所,武汉430064 [3]襄阳市农业科学院,湖北襄阳441057

出　　处：《东北农业科学》2022年第5期111-115,共5页Journal of Northeast Agricultural Sciences

基　　金：湖北省科学技术重大创新专项(2018ABA085);湖北省自然科学基金(2017CFB234)。

摘　　要：为了更准确、高效地揭示小麦不同器官的叶绿素含量,本研究以小麦的旗叶、小花和花药为材料,分别采用80%丙酮研磨法(Arnon法)、丙酮乙醇研磨法、丙酮乙醇浸提法来提取小麦不同组织器官的叶绿素。结果表明:不同提取方法提取的叶绿素溶液的吸收光谱基本一致,但在提取效率上存在差异,其中丙酮乙醇研磨法为旗叶和小花叶绿素提取的最佳方法,而用丙酮乙醇浸提法(浸提18 h)提取小麦花药的效率更高,这为准确提取和检测小麦不同组织器官叶绿素提供了必要的技术支持。In order to reveal the chlorophyll content of different organs of wheat more accurately and efficiently,the flag leaf,floret and anther of wheat were used as materials in this study to extract chlorophyll from different tissues and organs of wheat by using 80%acetone grinding method(Arnon method),acetone ethanol grinding method,and acetone ethanol extraction method respectively.The results showed that the absorption spectra of chlorophyll solutions extracted by different extraction methods were basically the same,but there were differences in extraction efficiency.Among them,the acetone ethanol grinding method was the best method for extracting chlorophyll from flag leaves and florets,while the acetone ethanol extraction method(extraction for 18 hours)was more efficient for extracting wheat anthers,which provided necessary technical support for accurate extraction and detection of chlorophyll from different tissues and organs of wheat.

关 键 词：小麦 旗叶 小花 花药 叶绿素含量 

分 类 号：S512.1[农业科学—作物学]