胰胆管注射牛磺胆酸钠及胰蛋白酶建立犬急性胰腺炎模型研究  

A dog model of acute pancreatitis established by injection of sodium taurocholate and trypsin into the pancreaticobiliary duct

作　　者：李美林 秦瑶 刘梦 金娟 任远 葛延松 郑家三[1] LI Meilin;QIN Yao;LIU Meng;JIN Juan;REN Yuan;GE Yansong;ZHENG Jiasan(College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《黑龙江畜牧兽医》2022年第22期70-74,140,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：黑龙江省博士后资助经费项目(LBH-Z19088);深圳市瑞鹏公益基金会高校青年教师(小动物临床)科研基金项目(RPJJ2020009)。

摘　　要：为了建立犬急性胰腺炎(acute pancreatitis,AP)模型,试验将10只成年健康犬随机分为两组,分别为模型组和对照组,每组5只。模型组于十二指肠小乳头处向胰胆管内逆行注射5%牛磺胆酸钠(按体重0.1 mL/kg)和胰蛋白酶(按体重3500 U/kg)混合溶液建立犬AP模型,对照组注射等量的生理盐水,术后24 h进行疼痛评分;并与自然发病组(收集的5只AP自然发病犬)比较,分析临床症状、影像学检查和实验室检查结果的差异。结果表明:术后24 h模型组出现明显的疼痛反应,疼痛评分为(13.00±5.14)分,显著高于对照组的(4.00±2.20)分(P<0.05),自然发病组和模型组均出现腹痛、腹泻、呕吐、无食欲、精神沉郁症状,B型超声波检查结果显示胰腺体积增大、回声不均匀。自然发病组的血红蛋白浓度、白细胞数、中性粒细胞数及血细胞比容高于参考值,其中血红蛋白浓度显著高于对照组(P<0.05);模型组的白细胞数、中性粒细胞数高于参考值。三组间白细胞数、中性粒细胞数均差异不显著(P>0.05)。模型组和自然发病组血清淀粉酶、血清脂肪酶活性及胆固醇含量显著或极显著高于对照组(P<0.05或P<0.01),模型组总蛋白含量极显著低于对照组(P<0.01),自然发病组血糖含量极显著高于对照组(P<0.01),模型组显著低于对照组(P<0.05),三组其余各项指标均差异不显著(P>0.05)。模型组与自然发病组犬胰腺特异脂肪酶检测结果均显示阳性,对照组显示阴性。说明通过手术进行胰胆管逆行注射药物成功建立犬胰腺炎模型。In order to establish a dog model of acute pancreatitis(AP),10 adult healthy dogs were randomly divided into two groups,the model group and the control group,with 5 dogs in each group.In the model group,a mixed solution of 5%sodium taurocholate(0.1 mL/kg by body weight)and trypsin(3500 U/kg by body weight)was retrogradely injected into the pancreaticobiliary duct at the small duodenal papilla to establish a canine AP model;the control group was injected with the same amount of normal saline.Pain score was performed 24 hours after operation.And compared with the natural onset group(collected 5 AP natural onset dogs),differences in clinical symptoms,imaging findings and laboratory tests were analyzed.The results showed that the model group had obvious pain response 24 hours after operation,and the pain score was(13.00±5.14)points,which was significantly higher than that of the control group(4.00±2.20)points(P<0.05).Dogs in both the natural onset group and the model group developed symptoms of abdominal pain,diarrhea,vomiting,loss of appetite,and lethargy;B-mode ultrasonography revealed pancreatic edema and uneven echogenicity.The hemoglobin concentration,leukocyte count,neutrophil count and hematocrit of the natural onset group were higher than the reference values,with the hemoglobin concentration significantly higher than that of the control group(P<0.05);the leukocyte count and neutrophil count of the model group were higher than the reference values.The differences in leukocyte count and neutrophil count among the three groups were not significant(P>0.05).Serum amylase,serum lipase activity and cholesterol levels were significantly or highly significantly higher in the model and natural onset groups than those in the control group(P<0.05 or P<0.01);total protein levels were highly significantly lower in the model group than in the control group(P<0.01);blood glucose levels were highly significantly higher in the natural onset group than in the control group(P<0.01),and significantly lower in the model group t

关 键 词：犬 急性胰腺炎 胰胆管逆行注射法 犬胰腺特异脂肪酶 血清 淀粉酶 脂肪酶 

分 类 号：S856.5[农业科学—临床兽医学]