Integrin β2 and β3:Two plasmatocyte markers deepen our understanding of the development of plasmatocytes in the silkworm Bombyx mori  

作　　者：Kui Zhang Jingjing Su Xin Hu Xiaomin Yan Siyuan Chen Chongyang Li Guangzhao Pan Hongbo Chang Wenli Tian Muhammad Nadeem Abbas Hongjuan Cui 

机构地区：[1]State Key Laboratory of Silkworm Genome Biology,College of Sericulture,Textile and Biomass Sciences,Southwest University,Chongqing,China [2]Chongqing iCELL Biotechnology Co.Ltd,Chongqing,China [3]Department of Gastroenterology,The Second Affliated Hospital of Chongqing Medical University,Chongqing,China [4]Institute of Apicultural Research,Chinese Academy of Agricultural Sciences,Bejing,China [5]Cancer Center,Reproductive Medicine Center,Medical Research Institute,Southwest University,Chongqing.China

出　　处：《Insect Science》2022年第6期1659-1671,共13页昆虫科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(31802142);the Doctoral Start-up Fund of the Southwest University(SWU120019);the F undamental Research Funds for the Central Universi-ties(XDJK2019C089);the project funded by the China Postdoctoral Science Foundation(2019T120801 and 2017M620408).

摘　　要：Insect hemocytes play important biological roles at developmental stages,metamorphosis,and innate immunity.As one of the most abundant cell types,plasmatocytes can participate in various innate immune responses,especially in encapsulation and node formation.Here,2 molecular markers of plasmatocytes,consisting of integrinβ2 andβ3,were identified and used to understand the development of plasmatocytes.Plasmatocytes are widely distributed in the hematopoietic system,including circulating hemolymph and hematopoietic organs(HPOs).HPOs constantly release plasmatocytes with high proliferative activity in vitro;removal of HPOs leads to a dramatic reduction in the circulating plasmatocytes,and the remaining plasmatocytes gradually lose their ability to proliferate in vivo.Our results demonstrated that the release of plasmatocytes from HPOs is regulated by insulin-mediated signals and their downstream pathways,including PI3K/Akt and MAPK/Erk signals.The insulin/PI3K/Akt signaling pathway can significantly irritate the hematopoiesis,and its inhibitor LY294002 could inhibit the hemocytes discharged from HPOs.While the insulin/MAPK/Erk signaling pathway plays a negative regulatory role,inhibiting its activity with U0126 can markedly promote the discharge of plasmatocytes from HPOs.Our results indicate that the circulating plasmatocytes are mainly generated and discharged by HPOs.This process is co-regulated by the PI3K/Akt and MAPK/Erk signals in an antagonistic manner to adjust the dynamic balance of the hemocytes.These findings can enhance our understanding of insect hematopoiesis.

关 键 词：Bombyx mori HEMATOPOIESIS MAPK/ERK PI3K/Akt plasmatocytes 

分 类 号：Q96[生物学—昆虫学]