wbnH2基因对北京欧文氏菌生物学特性及致病力的影响  

Effects of wbnH2 on biological characteristics and pathogenicity of Erwinia beijingensis

作　　者：常晓宁 刘宇[1] 谷彤彤 郭金英 宋忠娟 荣成博 CHANG Xiaoning;LIU Yu;GU Tongtong;GUO Jinying;SONG Zhongjuan;RONG Chengbo(Beijing Engineering Research Center for Edible Mushroom,Institute of Plant Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;School of Landscape and Ecological Engineering,Hebei University of Engineering,Handan 056038,Hebei,China)

机构地区：[1]北京市农林科学院植物保护研究所北京市食用菌工程技术研究中心,北京100097 [2]河北工程大学园林与生态工程学院,河北邯郸056038

出　　处：《微生物学通报》2022年第11期4713-4726,共14页Microbiology China

基　　金：北京市自然科学基金面上项目(6202008);北京市农林科学院创新能力建设专项(KJCX20200105);现代农业产业技术体系(CARS-20);邯郸市科学技术研究与发展计划项目(21422012325)。

摘　　要：【背景】北京欧文氏菌(Erwinia beijingensis)引起的刺芹侧耳细菌性软腐病(bacterial soft rot)给企业带来了严重的经济损失。wbnH2糖基转移酶基因在北京欧文氏菌中的生物学功能尚不明确。【目的】构建wbnH2基因的缺失株Δ-wbnH2和回补株C-wbnH2,探究wbnH2基因对北京欧文氏菌致病性的影响。【方法】采用同源重组方法构建北京欧文氏菌LMG 27579^(T) wbnH2基因缺失突变株Δ-wbnH2,并对基因缺失菌株的致病性、生长速度、运动能力、生物膜形成能力、黏附能力等生物学特性与野生型菌株进行比较分析;采用广宿主质粒pBBR1MCS2构建回补株C-wbnH2,排除了极性效应引起的突变株表型变化。【结果】与野生型菌株相比,基因缺失株Δ-wbnH2的生长速度无明显差别。但是wbnH2基因的缺失导致多糖分泌、生物膜形成能力、黏附能力、致病能力明显下降。【结论】wbnH2基因影响北京欧文氏菌多糖分泌、生物膜的形成能力、黏附能力及致病力,表明该基因在北京欧文氏菌致病过程中起重要作用,本研究为软腐病的防控提供了理论基础。[Background]Bacterial soft rot caused by Erwinia beijingensis results in serious economic losses to enterprises.The biological function of the glycosyltransferase gene wbnH2 in E.beijingensis remains unclear.[Objective]To explore the effect of wbnH2 gene on the pathogenicity of E.beijingensis.[Methods]Homologous recombination was employed to construct the E.beijingensis LMG 27579^(T) wbnH2-deleted mutantΔ-wbnH2.The biological characteristics such as pathogenicity,growth rate,motility,biofilm formation,and adhesion ofΔ-wbnH2 were studied.Further,we used the broad-host-range plasmid pBBR1MCS2 to construct the complementation strain C-wbnH2 for excluding the polarity effect-caused phenotypic change of the mutant.[Results]Compared with that of the wild type,the growth rate ofΔ-wbnH2 had no significant change.However,the deletion of wbnH2 resulted in significant decreases in polysaccharide secretion,biofilm formation,adhesion and pathogenicity.[Conclusion]The glycosyltransferase gene wbnH2 affects the polysaccharide secretion,biofilm formation,adhesion,and pathogenicity of E.beijingensis,playing a role in the pathogenic process.This study provides a theoretical basis for the prevention and control of bacterial soft rot.

关 键 词：刺芹侧耳 同源重组 软腐病 欧文氏菌 

分 类 号：S432.4[农业科学—植物病理学]