The plant circadian clock regulates autophagy rhythm through transcription factor LUX ARRHYTHMO  

作　　者：Ming-Kang Yang Xiao-Jie Zhu Chu-Min Chen Xu Guo Shu-Xuan Xu Ya-Rou Xu Shen-Xiu Du Shi Xiao Bernd Mueller-Roeber Wei Huang Liang Chen 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro‐Bioresources,College of Life Sciences,South China Agricultural University,Guangzhou 510642,China [2]Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou 510642,China [3]Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms,South China Agricultural University,Guangzhou 510642,China [4]State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources,School of Life Sciences,Sun Yat‐Sen University,Guangzhou 510275,China [5]Department of Molecular Biology,Institute of Biochemistry and Biology,University of Potsdam,14476 Potsdam‐Golm,Germany [6]Max Planck Institute of Molecular Plant Physiology,14476 Potsdam‐Golm,Germany

出　　处：《Journal of Integrative Plant Biology》2022年第11期2135-2149,共15页植物学报（英文版）

基　　金：the University of Potsdam;the Max Planck Institute of Molecular Plant Physiology for support;supported by the Laboratory of Lingnan Modern Agriculture Project(NZ2021001);the Natural Science Foundation of Guangdong Province(2022A1515011027,2021A1515012148,2019A1515012009);the Key Realm R&D Program of Guangdong Province(2020B0202090001);the Science and Technology Project of Guangzhou City(202102020907)。

摘　　要：Autophagy is an evolutionarily conserved degradation pathway in eukaryotes;it plays a critical role in nutritional stress tolerance.The circadian clock is an endogenous timekeeping system that generates biological rhythms to adapt to daily changes in the environment.Accumulating evidence indicates that the circadian clock and autophagy are intimately interwoven in animals.However,the role of the circadian clock in regulating autophagy has been poorly elucidated in plants.Here,we show that autophagy exhibits a robust circadian rhythm in both light/dark cycle(LD)and in constant light(LL)in Arabidopsis.However,autophagy rhythm showed a different pattem with a phase-advance shift and a lower amplitude in LL compared to LD.Moreover,mutation of the transcription factor LUX ARRHYTHMO(LUX)removed autophagy rhythm in LL and led to an enhanced amplitude in LD.LUX represses expression of the core autophagy genes ATG2,ATG8 a,and ATG11 by directly binding to their promoters.Phenotypic analysis revealed that LUX is responsible for improved resistance of plants to carbon starvation,which is dependent on moderate autophagy activity.Comprehensive transcriptomic analysis revealed that the autophagy rhythm is ubiquitous in plants.Taken together,our findings demonstrate that the LUXmediated circadian clock regulates plant autophagy rhythms.

关 键 词：AUTOPHAGY carbon starvation circadian clock LUX RHYTHM 

分 类 号：Q943.2[生物学—植物学]