白细胞分化抗原74在胆囊癌中的表达及其与表皮生长因子受体表达的相关性分析  

The expression of the cluster of differentiation 74 in tissues of gallbladder cancer and its correlation of the expression of EGFR

作　　者：王鹏[1] 邱振东 张利龙 左腾[1] 李凯 陈辰[1] 王卫星[1] WANG Peng;QIU Zhendong;ZHANG Lilong(Department of Generalsurgery,Renmin Hospital of Wuhan University,Wuhan 430000,China)

机构地区：[1]武汉大学人民医院普通外科,430000

出　　处：《临床外科杂志》2022年第10期977-980,共4页Journal of Clinical Surgery

摘　　要：目的分析白细胞分化抗原74(cluster of differentiation 74,CD74)分子在胆囊癌组织中的表达水平,并对其与胆囊癌组织中表皮生长因子受体(epidermal growth factor receptor,EGFR)的表达进行相关性分析。方法采用免疫组织化学方法对54例胆囊癌病人的组织标本进行分析,测定CD74及EGFR在不同分化程度胆囊癌组织中的表达情况,并对CD74在胆囊癌中的表达与临床病理特征进行分析,利用新鲜组织标本采用蛋白印迹法(Western blot,WB)测定新鲜组织中CD74的表达情况,通过Spearman秩相关分析CD74与EGFR表达的相关性。结果在54例胆囊癌病人中,CD74的表达与性别、年龄无明显相关(P>0.05)。分化差的胆囊癌中CD74的表达高于中分化及高分化胆囊癌(P<0.05),浸润深度高的胆囊癌组织中CD74的表达高于浸润深度低的胆囊癌组织(P<0.05),但与肿瘤组织的N分期无明显差异(P>0.05),TNM分期较早的胆囊癌与分期较差的胆囊癌组织中CD74的表达有明显差异(P<0.05)。通过Spearman秩相关分析CD74的表达与EGFR表达的相关性可以得出,CD74的IHC的IOD值与EGFR的IOD值呈正相关。结论CD74在分化差的胆囊癌中呈高表达,且与EGFR呈正相关。Objective To study and analyze the expression of the cluster of differentiation 74,CD74 in tissues of gallbladder cancer and its correlation of the expression of EGFR.Methods We used the immunohistochemistry to analyze the expressions of CD74 and EGFR in tissues of gallbladder cancer from 54 patients.The clinicopathological characters of gallbladder cancer patients and the expression of CD74 was also studied.We used the western blot to analyze the expression of CD74 in the fresh gallbladder cancer tissues.The Spearman rank correlation was used to analyze the association between CD74 and EGFR.Results The expression of CD74 had no difference between men and women,and the expression of CD74 had no correlation with the age of patients(P>0.05).The expression of CD74 in poorly differentiated gallbladder cancer tissues was significantly higher than that in moderately and well differentiated gallbladder cancer(P<0.05).The expression of CD74 with highly invasion depth was significantly higher than that in low invasion depth(P<0.05),but there was no significant difference with the N stage of tumor tissue(P>0.05).There was significant difference in the expression of CD74 between gallbladder carcinoma with early TNM stage and gallbladder carcinoma with poor TNM stage(P<0.05).Spearman rank correlation analysis showed that the IOD value of IHC of CD74 was positively correlated with the IOD value of EGFR.Conclusion CD74 is highly expressed in poorly differentiated gallbladder cancer,and it is positively correlated with EGFR.It is a potential target for the treatment of gallbladder cancer.

关 键 词：胆囊癌 白细胞分化抗原74 表皮生长因子受体 相关性分析 

分 类 号：R735.8[医药卫生—肿瘤]