Periostin蛋白在食管鳞状细胞癌组织中表达及其与患者临床病理特征的相关性  

Expression of Periostin Protein in Esophageal Squamous Cell Carcinoma and Its Correlation with Clinical Pathological Characteristics

作　　者：李超 徐小玲 厉娟 赵艳 LI Chao;XU Xiao-ling;LI Juan(Rizhao Central Hospital,Rizhao Shandong 276800)

机构地区：[1]山东省日照市中心医院,山东日照276800

出　　处：《医学临床研究》2022年第11期1622-1625,共4页Journal of Clinical Research

摘　　要：【目的】探讨食管鳞状细胞癌组织中Periostin蛋白表达情况及其与患者病理特征的相关性。【方法】收集在本院行手术切除的118例食管鳞状细胞癌组织标本(观察组),对应癌旁的正常食管组织(距癌组织5 cm以上)作为对照组,采用免疫组化法、半定量RT-PCR法和Western blot法检测食管鳞状细胞癌组织及癌旁正常食管组织中Periostin蛋白表达情况,采用Kaplan-Meier生存曲线、Cox比例风险回归模型分析Periostin蛋白的表达及其与食管鳞状细胞癌患者预后的相关性。【结果】观察组Periostin蛋白高表达率为63.6%(75/118),显著高于对照组的27.1%(32/118),差异有统计学意义(χ2=31.614,P<0.001)。Periostin mRNA在食管鳞状细胞癌组织中Periostin/GAPDH值高于正常食管组织,差异有统计学意义(P<0.05)。Periostin蛋白在正常食管癌旁组织的Periostin/β-actin值低于食管鳞状细胞癌组织,差异有统计学意义(P<0.05)。不同Periostin表达水平的食管鳞状细胞癌患者TNM分期、分化程度及淋巴结转移情况比较,差异有统计学意义(P<0.05)。Periostin低表达患者的生存率明显高于Periostin高表达患者(P<0.05)。Logistic多因素回归分析显示,Priostin高表达是影响食管鳞状细胞癌患者总生存期的危险因素(P<0.05)。【结论】Periostin在食管鳞状细胞癌组织中的表达与组织学类型、TNM分期及淋巴结转移有关,其预测食管鳞状细胞癌患者预后具有重要意义。【Objective】To investigate the expression of Periostin protein in esophageal squamous cell carcinoma and its correlation with the pathological features of patients.【Methods】A total of esophageal squamous cell carcinoma tissues(observation group)resected surgically in our hospital were collected,and the corresponding normal esophageal tissues(more than 5 cm from the cancer tissues)adjacent to the cancer were used as the control group.Immunohistochemistry,semi quantitative RT-PCR and Western blot were used to detect the expression of Periostin protein in esophageal squamous cell carcinoma tissues and normal esophageal tissues adjacent to the cancer.Kaplan-Meier survival curve Cox proportional hazard regression model was used to analyze the expression of Periostin protein and its correlation with the prognosis of patients with esophageal squamous cell carcinoma.【Results】The high expression rate of Periostin protein in the observation group was 63.6%(75/118),which was significantly higher than that in the control group[27.1%(32/118)].The difference was statistically significant(χ2=31.614,P<0.001).The value of Periostin mRNA in esophageal squamous cell carcinoma tissues was higher than that in normal esophageal tissues(P<0.05).The value of Periostin/β-actin in normal tissues adjacent to esophageal carcinoma was lower than that in esophageal squamous cell carcinoma(P<0.05).The TNM stage,differentiation degree and lymph node metastasis of esophageal squamous cell carcinoma patients with different Periostin expression levels were statistically significant(P<0.05).The survival rate of patients with low expression of Periostin was significantly higher than that of patients with high expression of Periostin(P<0.05).Logistic multivariate regression analysis showed that the high expression of Priostin was a risk factor affecting the overall survival of patients with esophageal squamous cell carcinoma(P<0.05).【Conclusion】The expression of Periostin in esophageal squamous cell carcinoma is related to histologic

关 键 词：食管肿瘤/病理学 癌 鳞状细胞 肿瘤蛋白质类 

分 类 号：R735.1[医药卫生—肿瘤]