阿尔茨海默病大鼠模型建立与磁共振成像  

Rat model of Alzheimer's disease and magnetic resonance imaging

作　　者：尹汇敏 郭宏[2] 赵文静 韩晓楠 金淼 崔红升 程浩 王玥 孟鑫 YIN Huimin;GUO Hong;ZHAO Wenjing;HAN Xiaonan;JIN Miao;CUI Hongsheng;CHENG Hao;WANG Yue;MENG Xin(College of Medical Technology,Qiqihar Medical College,Qiqihar,Heilongjiang 161006,China;Department of Radiology,Qiqihar First Hospital,Qiqihar,Heilongjiang 161006,China;Department of Magnetic Resonance,the Third Affiliated Hospital,Qiqihar Medical College,Qiqihar,Heilongjiang 161006,China;Medical Department,the Third Affiliated Hospital,Qiqihar Medical College,Qiqihar,Heilongjiang 161006,China)

机构地区：[1]齐齐哈尔医学院医学技术学院,黑龙江齐齐哈尔161006 [2]齐齐哈尔市第一医院放射科,黑龙江齐齐哈尔161006 [3]齐齐哈尔医学院附属第三医院磁共振科,黑龙江齐齐哈尔161006 [4]齐齐哈尔医学院附属第三医院医务科,黑龙江齐齐哈尔161006

出　　处：《中国医学工程》2022年第12期11-16,共6页China Medical Engineering

基　　金：黑龙江省齐齐哈尔市科学技术局联合引导科研项目(LHYD-2021062)。

摘　　要：目的通过D-半乳糖联合三氯化铝制备阿尔茨海默病(Alzheimer's disease,AD)大鼠模型的磁共振扫描(magnetic resonance imaging,MRI)提出适用于AD大鼠检测的医用磁共振线圈和序列。方法Morris水迷宫筛选24只健康SD大鼠并随机分为实验组和对照组,每组12只(雌雄各半)。实验组采用D-半乳糖联合三氯化铝造模,对照组以生理盐水代替D-半乳糖及三氯化铝以同样的建模方式注入。建模8周后进行糖水偏好实验、Morris水迷宫行为学测试,测试通过的实验组大鼠与对照组大鼠同时采用医用磁共振扫描(1.5 T Philips和3.0 T GE),磁共振数据经后台PACS系统上传至Philips Medical Systems工作站软件三维分析比较不同组大鼠脑结构和脑体积差异。结果与对照组比较,实验组大鼠体重减轻;糖水偏好百分比为:对照组(82±5.03)%,实验组为(63±12.67)%,两组差异有统计学意义(P<0.05);水迷宫测试结果显示:第6天潜伏期实验组大鼠为(15±14.3)s,对照组大鼠为(13±11.3)s,平台穿越次数实验组大鼠为(4±3.2)次,对照组大鼠为(5±2.6)次,两组差异有统计学意义(P<0.05);磁共振图像显示实验组大鼠脑沟增宽,脑皮质萎缩,脑体积减低。结论医用磁共振可用于AD大鼠监测诊断,为AD动物模型实验的进一步发展提供影像学诊断依据和参考思路。【Objective】Magnetic resonance imaging(MRI)coils and sequences were proposed for the detection of Alzheimer's disease(AD)in rats by magnetic resonance scanning of D-galactose combined with aluminum chloride.【Methods】Twenty-four healthy SD rats were screened by Morris water maze and randomly divided into experimental group and control group,with 12 rats in each group(half male and half female).The experimental group was modeled by D-galactose combined with aluminum trichloride,and the control group was injected with normal saline instead of D-galactose and aluminum trichloride in the same modeling way.After 8 weeks of modeling,the sugar water preference experiment and water maze behavior test were carried out.The rats in the experimental group and the rats in the control group underwent magnetic resonance scanning at the same time.The magnetic resonance data were uploaded to the Philips Medical Systems workstation software through the background PACS system,and the differences of brain structure and brain volume in different groups were compared.【Results】The results of sugar water preference experiment showed that compared with the control group,the rats in the experimental group lost weight,and the percentage of sugar water preference was 82%±5.03%in the control group and 63%±12.67%in the experimental group.The difference between the two groups was statistically significant(P<0.05).The results of water maze test showed that on the 6th day,the latency of rats in the experimental group was 15±14.3 s,13±11.3 s in the control group,4±3.2 times in the experimental group and 5±2.6 times in the control group.Magnetic resonance images showed that the cerebral sulcus of rats in the experimental group was widened,the cerebral cortex atrophied and the brain volume decreased compared with those in the control group.【Conclusion】MRI can provide a basis for the monitoring and diagnosis of AD rats,and this experiment can provide an imaging reference idea for the development of AD animal experiment.

关 键 词：阿尔茨海默病 大鼠模型 磁共振成像 脑结构与脑体积 

分 类 号：R749.16[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学] R445.2