传染性法氏囊病病毒超强毒株ZHA001株的分离及鉴定  

Isolation and Identification of Very Virulent Infectious Bursal Disease Virus Strain ZHA001

作　　者：于雷 潘雨 高洁 迟鑫 刘硕 YU Lei;PAN Yu;GAO Jie;CHI Xin;LIU Shuo(Beijing Zhonghai Biotic Co.Ltd,Beijing 100081,China)

机构地区：[1]北京中海生物科技有限公司,北京100081

出　　处：《中国兽药杂志》2022年第10期18-24,共7页Chinese Journal of Veterinary Drug

摘　　要：为研究某817肉鸡群发生传染性法氏囊病(IBD)原因,从病料中进行病毒分离,通过RT-PCR扩增分离株VP2基因后进行相关分析,并对分离株的致病性进行测定。结果显示,分离到一株传染性法氏囊病病毒(IBDV),将其命名为ZHA001株;ZHA001株VP2基因序列与超强毒株(vvIBDV)的同源性为97.5%~99.1%,氨基酸序列同源性大于99.3%;具有超强毒株的特征性氨基酸位点;遗传进化分析发现,ZHA001株属于超强毒株分支;致病性试验中,发病率为100%,致死率为90%。试验结果表明引起该鸡群IBD的病原为超强毒病毒,致病力强。To elucidate the reasons of infectious bursal disease(IBD)infection in the 817 broiler flocks,virus isolation was performed,the VP2 gene was amplified and determined,and the pathogenicity evaluation of isolation were carried out.The results showed that an infectious bursal disease virus(IBDV)strain designated as ZHA001 was isolated.The VP2 gene sequence of ZHA001 had 97.5%to 99.1%homology with the reference very virulent IBDV(vvIBDV)strains,and the homology of the amino acid sequence was above 99.3%with the vvIBDV strains.The VP2 amino acid sequence of IBDV ZHA001 strain was consistent with the characteristics of other vvIBDV strains.Phylogenetic analysis showed that ZHA001 belonged to the branch of vvIBDV strains.The mortality and morbidity of the infected SPF chickens was 90%and 100%.The results showed that the layers were infected with very virulent strain of IBDV,with high pathogenicity.

关 键 词：传染性法氏囊病病毒 VP2基因 序列分析 超强毒株 致病性 

分 类 号：S852.65[农业科学—基础兽医学]