藏柳茶多糖的提取、结构解析及抗氧化活性分析  

Extraction,Structure,and Antioxidant Activity Analysis of Polysaccharides from Sibiraea angustata

作　　者：杨许花 张竞文 刘红海[3] 高丹丹[1,2] 丁功涛 宋礼 马姝雯[1] YANG Xuhua;ZHANG Jingwen;LIU Honghai;GAO Dandan;DING Gongtao;SONG Li;MA Shuwen(College of Life Science and Engineering,Northwest Minzu University,Lanzhou 730124,China;Biomedical Research Center,Northwest Minzu University,Lanzhou 730300,China;Tobacco Industry Company,Lanzhou 730050,China;Gannan Research Institute of Yak Milk,Gannan 747000,China)

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730124 [2]西北民族大学生物医学研究中心,甘肃兰州730030 [3]甘肃烟草工业有限责任公司,甘肃兰州730050 [4]甘南牦牛乳研究院,甘肃甘南747000

出　　处：《现代食品科技》2022年第12期318-328,共11页Modern Food Science and Technology

基　　金：中央高校基本科研业务费资助项目(31920220024,31920210001);西北民族大学本科生创新项目(XBMU21139);中央高校基本科研业务费专项资金资助研究生项目(Yxm-2021073)。

摘　　要：该研究以藏柳茶为原料,用聚乙二醇-超声酶法辅助提取其粗多糖,采用响应面法优化其提取工艺参数。进一步用高效凝胶渗透色谱(HPGPC)高效液相色谱(HPLC)红外光谱(FT-IR)和扫描电镜(SEM)对藏柳茶多糖(SAPs)的结构进行初步解析,并考察其抗氧化活性。聚乙二醇-超声酶法辅助提取SAPs的最佳工艺参数为:PEG-400浓度为30%、酶添加量2%(m/m)、pH值4.0、超声功率80%、超声时间2.0h、超声温度80℃。此条件下,藏柳茶多糖提取率为10.95%。经HPLC分析,SAPs主要由半乳糖(13.34%)、葡萄糖(46.76%)、鼠李糖(8.78%)、果糖(2.89%)和阿拉伯糖(11.79%)组成;FT-IR及SEM分析表明,SAPs是一种β-糖苷键的吡喃型非晶态多糖结构。此外,SAPs体外抗氧化活性结果表明其DPPH·清除能力、·OH清除能力和·O_(2)清除能力的IC_(50)值分别为0.42、2.08、0.28mg/mL,且随着多糖含量的提高,其自由基清除能力提高,呈现剂量效应关系。所得提取工艺设计空间稳定可行,为藏柳茶多糖提取和产品的综合开发利用提供理论技术支持依据。Crude polysaccharides from Sibiraea angustata as the raw material were extracted using the polyethylene glycol-ultrasonic enzymatic method,and the extraction process parameters were optimized using response surface methodology.Furthermore,high-performance gel-permeation chromatography(HPGPC),high-performance liquid chromatography(HPLC),Fourier-transform infrared spectroscopy(FT-IR),and scanning electron microscopy(SEM)were used to characterize the structure of S.angustata polysaccharides(SAPs)and investigate their antioxidant activity.The optimal process parameters for SAP extraction assisted by the polyethylene glycol-ultrasonic enzymatic method were as follows:30%PEG-400 concentration,2%enzyme addition,pH 4.0,80%ultrasonic power,2 h ultrasonic treatment duration,and 80℃ultrasonic treatment temperature.Under these conditions,the extraction rate of polysaccharides was 10.95%.HPLC showed that the SAPs were mainly composed of galactose(13.34%),glucose(46.76%),rhamnose(8.78%),fructose(2.89%),and arabinose(11.79%).FT-IR and SEM revealed that SAPs are pyrenoid amorphous polysaccharide structures withβ-glycosidic bonds.In addition,analysis of the in vitro antioxidant activity of SAPs showed that the free radical scavenging ability increased with polysaccharide content in a dose-dependent manner.The IC_(50) values for DPPH,OH,and O_(2)free radicals were 0.42,2.08,and 0.28 mg/mL,respectively.The optimized extraction process is stable and feasible,providing a theoretical and technical basis for the extraction of SAPs from S.angustata and the comprehensive development and utilization of these products.

关 键 词：藏柳茶 多糖 响应面 结构解析 抗氧化 

分 类 号：TS272.5[农业科学—茶叶生产加工]