慢性牙周炎相关差异表达基因谱筛选及其与病情严重程度的相关性  

Differentially expressed genes in chronic periodontitis and the correlation with disease severity

作　　者：潘裕兴 张欢 李世英[1] 王艳华[1] 马瑾 王天 Pan Yuxing;Zhang Huan;Li Shiying;Wang Yanhua;Ma Jin;Wang Tian(Department of Stomatology,Tianjin People's Hospital,Tianjin 300122,China;Department of Stomatology,Women and Children's Health Care and Family Planning Service Center,Tianjin 300121,China)

机构地区：[1]天津市人民医院口腔科,天津300122 [2]天津市红桥区妇女儿童保健和计划生育服务中心口腔科,天津300121

出　　处：《国际生物医学工程杂志》2022年第2期136-141,共6页International Journal of Biomedical Engineering

基　　金：天津市人民医院院级课题(2019YJ004)。

摘　　要：目的研究慢性牙周炎(CP)的差异表达基因,探讨其与牙周炎程度的相关性。方法在高通量基因表达(GEO)数据库中筛选与CP相关的基因表达谱数据并用GEO2R在线软件进行分析,绘制火山图。对筛选出的CP相关差异表达基因进行京都基因与基因组百科全书(KEEG)、基因本体论(GO)分析,预测其可能功能及信号通路。采用蛋白-蛋白相互作用数据库(STRING)分析筛选出的CP相关差异表达基因的编码蛋白之间的相互作用关系。并用Cytohubba软件鉴定信号通路关键基因。选取120例CP患者和40名健康对照。采用实时聚合酶链锁反应(q-PCR)法对筛选出的CP相关基因进行验证。结果共筛选出符合要求的CP差异表达基因1151个。这些基因在GO通路上主要富集于粒细胞分化的正调控、辅助性T细胞分化、白细胞聚集、急性炎症反应调节、趋化因子介导和内质网未折叠蛋白反应等;在KEGG路上主要富集于NFB通路、趋化因子通路、细胞因子受体相互作用、白细胞跨内皮迁移通路、B细胞受体通路、Toll样受体通路等。使用筛选到的CP相关差异表达基因构建蛋白质间相互作用网络,共包括78个节点和496个链接,平均聚集系数和平均连接度分别为0.69和12.7。Cytohubba分析结果显示,Sell为信号通路的关键基因,其在不同程度的3个CP组中齿龈液的相对表达水平(1.14±0.46、0.86±0.41、0.52±0.46)显著低于对照组(1.50±0.65)(P<0.05)。用齿龈液中Sell表达水平诊断CP的受试者工作特征曲线下面积(AUC)为0.79(95%CI:0.71~0.86)。将Sell作为评价CP严重程度的生物学标志物时,95%CI条件下的AUC值均大于0.65。结论CP相关差异表达基因主要富集于牙周炎炎症反应相关的信号通路上。Sell基因在CP患者的齿龈沟液中的表达水平明显减低,并与病情严重程度有关。Sell基因有望成为CP分级的生物标志物。Objective To study the differentially expressed genes in chronic periodontitis(CP)and to explore the correlation with disease severity.Methods Gene expression profile data associated with CP were screened in the Gene Expression Omnibus(GEO)database and analyzed with GEO2R online software to create volcano maps.Kyoto Encyclopedia of Genes and Genomes(KEEG)and Gene Ontology(GO)analyses were performed on the screened CP-associated differentially expressed genes to predict their possible functions and signaling pathways.The protein-protein interaction database(STRING)was used to analyze the interaction relationships between the encoded proteins of the screened CP-related differentially expressed genes.Cytohubba software was used to identify key genes in the signaling pathway.One120 CP patients and 40 healthy controls were selected.The screened CP-related genes were validated by the real-time polymerase chain reaction(q-PCR)method.Results A total of 1151 CP differentially expressed genes that met the requirements were screened.These genes were mainly enriched in the GO pathway for positive regulation of granulocyte differentiation,helper T-cell differentiation,leukocyte aggregation,regulation of acute inflammatory response,chemokine-mediated and endoplasmic reticulum unfolded protein response,as well as in the KEGG pathway for NFB pathway,chemokine pathway,cytokine receptor interaction,leukocyte transendothelial migration pathway,B-cell receptor pathway,Toll-like receptor pathway,etc.The protein-protein interaction network was constructed using the screened CP-related differentially expressed genes,which included 78 nodes and 496 links,with a mean aggregation coefficient and mean connectivity of 0.69 and 12.7,respectively.Cytohubba analysis showed that Sell was a key gene in the signaling pathway,and its relative expression levels in the gingival fluids of the three CP groups with different degrees(1.14±0.46,0.86±0.41,0.52±0.46)was significantly lower than that of the control group(1.50±0.65)(all P<0.05).The area

关 键 词：慢性牙周炎 差异表达基因 信号通路 诊断 

分 类 号：R781.42[医药卫生—口腔医学]