鹅和鸡胚胎期皮肤毛囊中FGFR3基因的表达规律  

Expression of FGFR3 Gene in Embryonic Skin Follicles of Goose and Chicken

作　　者：宋玉朴 张磊 常晓飞 郭婵影 宗艳峰 隋玉健[1] 孙永峰[1] SONG Yupu;ZHANG Lei;CHANG Xiaofei;GUO Chanying;ZONG Yanfeng;SUI Yu-jian;SUN Yongfeng(College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;Jiangxi Agricultural Technology Extension Center,Nanchang 330046,China;Chang-chun BCHT Biotechnology Co.,Ltd.,Changchun 130012,China)

机构地区：[1]吉林农业大学动物科学技术学院,长春130118 [2]江西省农业技术推广中心,南昌330046 [3]长春百克生物科技股份公司,长春130012

出　　处：《吉林农业大学学报》2022年第5期607-612,共6页Journal of Jilin Agricultural University

基　　金：吉林省重点研发项目(20180201034NY);吉林省中青年科技创新领军人才及团队项目(20200301035RQ);吉林省畜牧业管理局畜禽遗传资源开发利用项目(2020051000001);吉林省畜牧业管理局吉林省畜牧科技提质增效技术示范推广项目(202003);吉林农业大学2019年本科生科技创新基金项目;吉林省教育厅产业化重点项目(JJKH20210376KJ);吉林省科技引导计划项目(科特派20191001001XH)。

摘　　要：以籽鹅和“京白1号”鸡为研究对象,利用苏木精-伊红染色法(HE染色)对皮肤组织进行常规染色观察。采用荧光定量PCR和蛋白免疫印迹技术(Western blot)研究FGFR3基因mRNA及其目的蛋白在鹅、鸡胚胎期3个重要发育时间节点(鹅胚胎期13,18,28 d和鸡胚胎期9,12,18 d)表达趋势。荧光定量PCR结果显示:FGFR3基因mRNA表现为伴随鹅皮肤毛囊发育而表达量显著上升的趋势(P<0.05),而“京白1号”鸡则表现出相反的表达趋势。通过WB试验结果可以得知,目的蛋白表达量均随毛囊的生长发育而显著降低。结果表明:FGFR3可能参与到毛囊生长发育调控,表现出在毛囊分化过程中的促分化作用。Zi goose and"Jingbai 1"chicken were used for routine observation of skin tissue by hematoxylin and HE staining.The expression trends of FGFR3 mRNA and its target proteins at three important developmental time nodes(goose embryonic day 13,18,28 and chicken embryonic day 9,12,18)in goose and chicken were investigated by quantitative PCR and Western-blot.The expression of FGFR3 mRNA showed a significant trend(P<0.05)with follicle development in goose skin,but the opposite trend was observed in“Jingbai1”chicken.As expected from the WB results,the target protein expression all decreased significantly with hair follicle growth and development.Taken together,FGFR3 may be involved in the regulation of hair follicle growth and development,showing a pro differentiation role during hair follicle differentiation.

关 键 词：FGFR3 籽鹅 “京白1号”鸡 毛囊 WESTERN-BLOT 实时荧光定量PCR 

分 类 号：S83[农业科学—畜牧学]