大豆蛋白源替代鱼粉对黄金鲫生长、蛋白质酶活力及免疫指标的影响  

Effects of Replacing Fish Meal with Different Soybean Protein Sources on Growth Performance,Protease Activities and Immune Indices of Carassius auratus

作　　者：杨智勇 杨兰 李登来 李良 朱瑞 于哲 吴莉芳[1] YANG Zhiyong;YANG Lan;LI Denglai;LI Liang;ZHU Rui;YU Zhe;WU Li-fang(College of Animal Science and Technology/College of Veterinary Medicine,Jilin Agricultural Uni-versity,Changchun 130118,China;Key Laboratory for Animal Production and Product Quality and Safety of the Ministry of Education,Changchun 130118,China;Key Laboratory of Animal Nu-trition and Feed Science of Jilin Province,Changchun 130118,China;Lotus Township People's Government of Shulan City,Shulan 132623,China)

机构地区：[1]吉林农业大学动物科学技术学院/动物医学院,长春130118 [2]动物生产及产品质量安全教育部重点实验室,长春130118 [3]吉林省动物营养与饲料科学重点实验室,长春130118 [4]吉林省舒兰市莲花乡人民政府,舒兰132623

出　　处：《吉林农业大学学报》2022年第5期624-630,共7页Journal of Jilin Agricultural University

基　　金：吉林省自然科学基金项目(20170101026JC)。

摘　　要：为研究膨化大豆粉、发酵豆粕以及豆粕对黄金鲫(Carassius auratus)生长、蛋白酶活力、蛋白质代谢酶活力及非特异性免疫指标的影响,以初始体质量为(43.31±2.11)g/尾的黄金鲫为研究对象。在黄金鲫的配合饲料中,以膨化大豆粉、发酵豆粕和豆粕分别替代60%的鱼粉,配制成4种等氮等脂肪的配合饲料,分别为对照组、膨化大豆粉组、发酵豆粕组和豆粕组,在室内控温单循环养殖系统中,进行为期8周的饲养试验。饲养试验结束后,采用福林-酚试剂法,测定蛋白酶活力;采用试剂盒测定肝胰脏中谷丙转氨酶、谷草转氨酶、碱性磷酸酶、超氧化物歧化酶和溶菌酶活力。结果表明:发酵豆粕组黄金鲫特定生长率高于豆粕组(P<0.05);发酵豆粕组黄金鲫蛋白质效率低于对照组(P<0.05);发酵豆粕组黄金鲫肝胰脏、前肠、中肠和后肠蛋白酶活力显著低于对照组(P<0.05);发酵豆粕组黄金鲫肝胰脏和后肠蛋白酶活力显著高于膨化大豆粉组和豆粕组(P<0.05);发酵豆粕组黄金鲫谷丙转氨酶活力低于对照组(P<0.05);发酵豆粕组黄金鲫碱性磷酸酶活力低于膨化大豆粉组(P<0.05);发酵豆粕组黄金鲫超氧化物歧化酶活力高于豆粕组(P<0.05)。黄金鲫配合饲料中膨化大豆粉、发酵豆粕和豆粕替代鱼粉比例为60%时,发酵豆粕替代鱼粉效果最好,膨化大豆粉次之,而豆粕的效果最差。In order to study the effects of expanded soybean meal,fermented soybean meal and soybean meal on the growth,protease activity,protein metabolism enzyme activity and non-specific immune indices of Carassius auratus,this experiment took Carassius auratus with an initial body weight of(43.31±2.11)g/tail as the research object.In the compound feed of Carassius auratus,expanded soybean meal,fermented soybean meal and soybean meal were used to replace 60%fish meal,and formulated into 4 kinds of compound feeds which were the control group(ck),expanded soybean meal group(ESBM),fermented soybean meal group(FSBM)and soybean meal group(SBM)with equal nitrogen and fats.An 8-week breeding trial was conducted in an indoor temperature-controlled single-cycle breeding system.After the feeding experiment,the protease activity was determined by the Folin-phenol reagent method,the activities of GPT,GOT,AKP,SOD and LZM in hepatopancreas were determined by Nanjing Jiancheng kit.These results show that the specific growth rate of Carassius auratus in the FSBM group was higher than that in the SBM group(P<0.05);the protein efficiency ratio of Carassius auratus in the FSBM group was lower than that in the ck group(P<0.05);the protease activity of Carassius auratus′s hepatopancrea,fore intestine,middle intestine and hind intestine in the FSBM group was lower than that in the ck group(P<0.05);the protease activity of Carassius auratus′s hepatopancrea and hind intestine of the FSBM group was higher than that of the ESBM group and SBM group(P<0.05);the GPT activity of Carassius auratus in the FSBM group was lower than that in the ck group(P<0.05);the AKP activity of Carassius auratus in the FSBM group was lower than that of the ESBM group(P<0.05);the SOD activity of Carassius auratus in the FSBM group was higher than that in the SBM group(P<0.05).In summary,under the experimental conditions,when the proportion of ESBM,FSBM and SBM replacing fish meal in Carassius auratus compound feed is 60%,the effect of FSBM replacing fish meal is t

关 键 词：黄金鲫 大豆蛋白源 鱼粉 蛋白质代谢酶 非特异性免疫指标 

分 类 号：S965.117[农业科学—水产养殖]