基于含量测定和指纹图谱的不同基原地骨皮药材异同点考察  

Chemical Consistency of Lycii Cortex from Different Origins Based on Component Contents and Fingerprint

作　　者：李宝鑫 郝丽霞 代云桃[1] 李西文[1] 邬兰[1] LI Baoxin;HAO Lixia;DAI Yuntao;LI Xiwen;WU Lan(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijng 100700,China)

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2022年第24期179-185,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院中药研究所古代经典名方的开发研究项目(H2018026)。

摘　　要：目的:通过比较2020年版《中华人民共和国药典》收载的不同基原地骨皮药材主要化学组成的异同点,判定2种基原的地骨皮是否具有化学一致性。方法:收集有代表性的30批地骨皮药材,采用超高效液相色谱-光电二极管阵列检测器法(UPLC-PDA)建立含量测定方法及指纹图谱分析方法,分别以乙腈(A)-0.15%三氟乙酸水溶液(B)和乙腈(A)-0.1%甲酸水溶液(B)作为流动相进行梯度洗脱(0~4 min,5%~12%A;4~8 min,12%A;8~12 min,12%~14%A;12~15 min,14%~30%A;15~17 min,30%~40%A;17~18 min,40%~90%A),检测波长280 nm。结合多元统计分析,评价不同基原地骨皮的异同点。结果:建立的含量测定及指纹图谱方法具有良好的精密度、稳定性和重复性。30批地骨皮的指纹图谱与对照指纹图谱的相似度均>0.9,且宁夏枸杞地骨皮和枸杞地骨皮指纹图谱中的8个相同共有峰均为酚酰胺类成分,分别为地骨皮乙素、N-(4,9,13-triazatridecan-1-yl)-3,4-dihydroxybenzenepropanamide、阿魏酰基-1,4-丁二胺、N_(1),N_(5)-bis(dihydrocaffeoyl)spermidine或N_(5),N_(10)-bis(dihydrocaffeoyl)spermidine、N_(5)-caffeoyl-N_(10)-dihydrocaffeoylspermidine、N_(5)-dihydrocaffeoyl-N_(10)-caffeoylspermidine、N_(1),N_(5)-bis(caffeoyl)spermidine和枸杞素A。其中地骨皮乙素在宁夏枸杞地骨皮、枸杞地骨皮中的质量分数范围分别为2.52%~12.24%、1.22%~8.18%。结论:建立的UPLC分析方法可用于地骨皮药材的含量测定和指纹图谱分析。宁夏枸杞地骨皮与枸杞地骨皮的化学轮廓相似,地骨皮乙素含量差异无统计学意义,二者化学组成基本一致。Objective:In this study,the two different origins of Lycii Cortex in the 2020 edition of Chinese Pharmacopoeia were determined to analyze their chemical consistency by comparing their main chemical composition.Method:Thirty representative batches of Lycii Cortex were collected,conten determination and fingerprint analysis methods were established by ultra performance liquid chromatographyphotodiode array detector(UPLC-PDA)combining with multivariate statistical analysis to evaluate the similarities and differences between two origins of Lycii Cortex.Respectively by the mobile phase of acetonitrile(A)-0.15%trifluoroacetic acid aqueous solution(B)and the mobile phase of acetonitrile(A)-0.1% formic acid aqueous solution(B)for gradient elution(0-4 min,5%-12%A;4-8 min,12%A;8-12 min,12%-14%A;12-15 min,14%-30%A;15-17 min,30%-40%A;17-18 min,40%-90%A),and the detection wavelength was se at 280 nm.Result:This established content determination and fingerprint methods had good precision,stability and repeatability.The similarities of 30 batches of Lycii Cortex were above 0.90 by comparing with the control fingerprint,and the eight common peaks in fingerprints of Lycii Cortex from Lycium barbarum and L.chinense were all phenolic amides,which were kukoamine B,N-(4,9,13-triazatridecan-1-yl)-3,4-dihydroxybenzenepropanamide,feruloylputrescine,N_(1),N_(5)-bis(dihydrocaffeoyl)spermidine or N_(5),N_(10)-bis(dihydrocaffeoyl)spermidine,N_(5)-caffeoyl-N_(10)-dihydrocaffeoylspermidine,N_(5)-dihydrocaffeoyl-N_(10)-caffeoylspermidine,N_(1),N_(5)-bis(caffeoyl)spermidine and lyciumin A.Among them,the content ranges of kukoamine B in Lycii Cortex from L.chinense and L.barbarum were 1.22%-8.18%,2.52%-12.24%,respectively.Conclusion:The established UPLC analysis method can be used for the content determination and fingerprint analysis of Lycii Cortex.This study indicates that chemical contour of Lycii Cortex from L.barbarum and L.chinense are similar,there are no significant differences in kukoamine B content,and they have consistency in the ch

关 键 词：地骨皮 药材基原 超高效液相色谱法(UPLC) 指纹图谱 地骨皮乙素 化学一致性评价 经典名方 

分 类 号：R22[医药卫生—中医基础理论] R28[医药卫生—中医学] R931[理学—分析化学] O657[理学—化学]