泛素化结合酶E2抑制剂对草菇15℃保鲜的影响  

Effects of inhibitor ubiquitin binding enzyme E2 on preservation of Volvariella volvacea at 15℃

作　　者：龚明[1] 尹昕 黄天宇 郑婷婷 唐利华[1] 张赫男[1] 汪滢[1] GONG Ming;YIN Xin;HUANG Tianyu;ZHENG Tingting;TANG Lihua;ZHANG Henan;WANG Ying(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Southern Key Laboratory of Edible Fungus Resource Utilization,Ministry of Agriculture and Rural Affairs,National Engineering Research Center of Edible Fungi,National R&D Center for Edible Fungal Processing,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China;College of Chemistry and Life Sciences,Changchun University of Technology,Changchun 130012,Jilin,China;College of Food Science and Engineering,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China;College of Food Science&Technology,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海市农业科学院食用菌研究所农业农村部南方食用菌资源利用重点实验室国家食用菌工程技术研究中心国家食用菌加工技术研发分中心上海市农业遗传育种重点开放实验室,上海201403 [2]长春工业大学化学与生命科学学院,吉林长春130012 [3]江苏海洋大学食品科学与工程学院,江苏连云港222005 [4]上海海洋大学食品学院,上海201306

出　　处：《菌物学报》2022年第12期2074-2082,共9页Mycosystema

基　　金：国家自然科学基金(32072642)。

摘　　要：为了改进草菇低温保鲜方法,在15℃贮藏条件下,采用泛素化结合酶E2(UBEV2)抑制剂对草菇子实体进行处理,并开展相关生理指标和基因表达检测。结果表明,使用100μmol/L的UBEV2抑制剂L345-0044维持了草菇较好的品质,提高了草菇可溶性糖的含量。低温明显提升抑制剂处理下的碱性蛋白酶和中性蛋白酶活力,并证实了低温胁迫显著提高了抑制剂处理下的一种类型蛋白酶肽基赖氨酸金属内肽酶的表达。本研究证实了草菇可溶性糖和高活性的冷诱导金属内肽酶对于延长草菇的低温保鲜时间是必须的。Fruiting bodies of Volvariella volvacea were treated with ubiquitin binding enzyme E2(UBEV2)inhibitor for improving the preservation quality at low temperature of 15℃,and related physiological indexes and gene expression were detected.The 100μmol/L UBEV2 inhibitor treatment maintained better quality of V.volvacea and increased the soluble sugar content of V.volvacea.Low temperature significantly increased alkaline protease activities and neutral protease activities under inhibitor treatment.It was confirmed that cold pressure significantly increased the expression of peptidyl-lysine metalloendopeptidase,a type of protease,under inhibitor treatment.This study demonstrated that the soluble sugar and high activities of cold-induced metalloendopeptidase were necessary for prolonging the storage time of V.volvacea at low-temperature.

关 键 词：草菇 泛素化结合酶E2 蛋白酶 金属内肽酶 低温保鲜 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]