环形交叉轴突导向受体同源物1在神经胶质瘤组织的表达及其临床意义  

Expression and clinical significance of circular chiasmatic axon guidance receptor homolog 1 in glioma

作　　者：岳四海 张宏凤[2] Yue Sihai;Zhang Hongfeng(Henan Provincial People’s Hospital,People’s Hospital of Zhengzhou University,People’s Hospital of Henan University,Zhengzhou 450003,China;Department of Neurosurgery,Zhengzhou Central Hospital,Zhengzhou 450003,China)

机构地区：[1]河南省人民医院,郑州大学人民医院,河南大学临床医学院神经外科,450000 [2]郑州市中心医院血液科,450000

出　　处：《中华实验外科杂志》2022年第11期2208-2210,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨环形交叉轴突导向受体同源物1(ROBO1)在神经胶质瘤组织表达和临床意义。方法选取2019年9月至2022年9月河南省人民医院收治的72例神经胶质瘤和癌旁组织作为研究对象,采用荧光定量和免疫组织化学分析ROBO1蛋白在神经胶质瘤和癌旁组织中的表达水平;并分析ROBO1表达水平与神经胶质瘤患者临床病理特征之间的关系。采用生存曲线分析ROBO1表达水平与患者预后预后的关系。并采用单因素方差分析和多因素Cox比例分线回归模型分析影响神经胶质瘤预后的独立危险因素。结果神经胶质瘤组织中ROBO1信使RNA(mRNA)(2.38±0.35)明显高于癌旁组织(1.04±0.19),差异有统计学意义(t=28.390,P<0.05)。神经胶质瘤组织中ROBO1蛋白表达阳性率(90.28%,65/72)明显高于癌旁组织(11.11%,8/72),差异有统计学意义(χ^(2)=25.321,P<0.05)。Ⅰ~Ⅱ级患者神经胶质组织ROBO1 mRNA水平(2.14±0.20)明显低于Ⅲ~Ⅳ级患者(2.60±0.32),差异有统计学意义(t=7.180,P<0.05)。淋巴结转移患者神经胶质组织ROBO1 mRNA水平(2.65±0.26)明显高于未淋巴结转移患者(2.09±0.12),差异有统计学意义(t=11.480,P<0.05)。KPS评分<80分患者神经胶质组织ROBO1 mRNA水平(2.67±0.25)明显高于KPS评分>80分患者(2.09±0.13),差异有统计学意义(t=12.410,P<0.05)。术后复发患者神经胶质组织ROBO1 mRNA水平(2.60±0.30)明显高于未复发患者(2.09±0.13),差异有统计学意义(t=8.817,P<0.05)。ROBO1高表达患者生存时间(15.6个月)明显低于ROBO1低表达患者(24.1个月),差异有统计学意义(Log-Rank=10.251,P<0.05)。ROBO1高表达患者术后3年生存率[21.25%(10/31)]低于ROBO1低表达组[73.17%(30/41)],差异有统计学意义(Log-Rank=8.014,P<0.05)。单因素方差分析显示,ROBO1 mRNA水平、高WHO分级、淋巴结转移、KPS评分和术后复发是影响脑胶质瘤患者预后的独立危险因素。结论ROBO1蛋白在神经胶质瘤细胞中表达显著上调,恶性程度和Objective To investigate the expression and clinical significance of roundabout guidance receptor 1(ROBO1)in glioma.Methods 72 cases of glioma and adjacent cancer tissues treated in our hospital from September 2019 to September 2022 were selected as research objects.The expression level of ROBO1 protein in glioma and adjacent cancer tissues was analyzed by fluorescence quantification and immunohistochemistry.The relationship between the expression level of ROBO1 and the clinicopathological characteristics of patients with glioma was analyzed.The survival curve was used to analyze the relationship between ROBO1 expression level and prognosis.Univariate ANOVA and Cox proportional regression model were used to analyze the independent risk factors affecting the prognosis of glioma.Results ROBO1 mRNA in glioma tissues(2.38±0.35)was significantly higher than that in adjacent tissues(1.04±0.19,t=28.390,P<0.05).The positive rate of ROBO1 protein expression in glioma tissues(90.28%,65/72)was significantly higher than that in adjacent tissues(11.11%,8/72,χ^(2)=25.321,P<0.05).The level of ROBO1 mRNA in glial tissue of patients with gradeⅠ-Ⅱ(2.14±0.20)was significantly lower than that of patients with gradeⅢ-Ⅳ(2.60±0.32),the difference was statistically significant(t=7.180,P<0.05).The level of ROBO1 mRNA in glial tissue of patients with lymph node metastasis(2.65±0.26)was significantly higher than that of patients without lymph node metastasis(2.09±0.12,t=11.480,P<0.05).The ROBO1 mRNA level in glial tissue of patients with KPS score<80(2.67±0.25)was significantly higher than that of patients with KPS score>80(2.09±0.13,t=12.410,P<0.05).ROBO1 mRNA level in recurrent patients was significantly higher than that in non recurrent patients(2.60±0.30,2.09±0.13,t=8.817,P<0.05).The survival time of patients with high expression of ROBO1(15.6 months)was significantly lower than that of patients with low expression of ROBO1(24.1 months,Log rank=10.51,P<0.05).The 3-year survival rate of patients with high ROBO1 expres

关 键 词：环形交叉轴突导向受体同源物1 神经胶质瘤 预后 淋巴结转移 

分 类 号：R739.4[医药卫生—肿瘤]