改良腹膜黏连大鼠动物模型的建立  

Establishment and evaluation of an improved rat model of peritoneal adhesion

作　　者：路小欢 徐鲁明 万超 郝璐 李孝琼 周铖 袁野[1] 王国斌[1] 王征[1] Lu Xiaohuan;Xu Luming;Wan Chao;Hao Lu;Li Xiaoqiong;Zhou Cheng;Yuan Ye;Wang Guobin;Wang Zheng(Department of Gastrointestinal Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China;Research Center for Tissue Engineering and Regenerative Medicine,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China)

机构地区：[1]华中科技大学同济医学院附属协和医院胃肠外科,武汉430022 [2]华中科技大学同济医学院附属协和医院组织工程与再生医学研究中心,武汉430022

出　　处：《中华实验外科杂志》2022年第11期2233-2236,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(82173315、81974382);湖北省技术创新专项重大项目(2018ACA136);中央高校基本科研业务费专项资金资助(2021yjsCXCY126);华中科技大学同济医学院重大疾病交叉创新团队培育计划。

摘　　要：目的构建一种改良腹膜黏连大鼠动物模型。方法48只Sprague-Dawley(SD)大鼠完全随机均分为假手术对照组和实验组。假手术对照组仅做剖腹探查。实验组开展改良盲肠-腹膜黏连造模,通过摩擦1 cm×2 cm范围内盲肠浆膜造成损伤,同时刮擦对侧腹壁形成1 cm×2 cm缺损,术中评价盲肠损伤程度。术后3、7、30 d分别剖腹探查腹膜黏连程度,黏连组织行苏木精-伊红(HE)及马松(Masson)染色。结果该模型构建平均时间25 min,术中盲肠出血点平均为(11.670±0.271)个。与假手术空白组比较,实验组术后3、7、30 d剖腹探查均可见盲肠与腹膜黏连。HE及马松染色显示黏连带中大量胶原纤维沉积。结论该改良方法为构建腹膜黏连大鼠动物模型提供稳定工具。Objective To establish an improved rat model of peritoneal adhesion.Methods 48 Sprague-Dawley rats were randomly divided into control group and experimental group.The rats in the control group only underwent exploratory laparotomy.In the experimental group,an improved rat model of cecum-abdominal wall adhesion was established by abrasing cecum serosa membrane within 1 cm×2 cm area and scraping the contralateral abdominal wall within 1 cm×2 cm area.The severity of cecum injury was evaluated during surgery.At the 3rd,7th and 30th day,the laparotomy was performed to evaluate the peritoneal adhesion and the adhesion area was measured.The histological evaluation of adhesion was performed by hematoxylin and eosin(HE)and Masson’s trichrome staining.Results The average operation time was 25 min,and the average number of intraoperative cecum bleeding spots were 11.670±0.271.Compared with the control group,cecum-abdominal wall adhesion was observed in the experimental group at the 3rd,7th and 30th day after operation.HE and Masson’s trichrome staining showed the formation of adhesion fusion and significant deposition of collagen fibers in the adhesion area.Conclusion This protocol provides a stable tool for developing rat model of peritoneal adhesion.

关 键 词：腹膜黏连 大鼠 动物模型 

分 类 号：R-332[医药卫生]