人源性类器官在结直肠癌中的应用及研究进展  

Application and research progress of patient derived organoids in colorectal cancer

作　　者：方效昌 李炫飞 冯茂辉[1] Fang Xiaochang;Li Xuanfei;Feng Maohui(Department of Gastrointestinal Surgery,Zhongnan Hospital of Wuhan University,Clinical Medical Research Center of Peritoneal Cancer of Wuhan,Clinical Cancer Study Center of Hubei Province,Key Laboratory of Tumor Biological Behavior of Hubei Province,Wuhan 430071,China)

机构地区：[1]武汉大学中南医院胃肠外科,武汉市腹膜癌临床医学研究中心,湖北省肿瘤医学临床研究中心,肿瘤生物学行为湖北省重点实验室,430071

出　　处：《中华实验外科杂志》2022年第11期2282-2285,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(82070302、1902018);武汉市腹膜癌临床医学研究中心资助项目(2015060911020462);武汉大学中南医院科技创新培育基金资助项目(CXPY2022055)。

摘　　要：类器官是一种新兴的三维培养模型,具有培养简单、稳定性好、能高度重现来源组织的特征和异质性等优点。它既可作为临床预测模型,在体外对患者进行抗癌药物筛选和敏感性测试,从而制定个性化的治疗方案;亦可以作为基础研究的工具,通过进行自身基因编辑和修饰研究,或结合气-液界面培养法和微流控技术研究,探索结直肠癌发生发展分子机制及寻找潜在治疗靶点。本文就结直肠癌类器官在临床应用和基础研究中的现状和进展作一综述。Organoid is a new three-dimensional culture model,which has the advantages of simple culture,good stability,highly reproducing the characteristics and heterogeneity of source tissues.It can be used as a clinical prediction model to screen anticancer drugs and test the sensitivity of patients in vitro,so as to formulate personalized treatment plans;It can also be used as a tool for basic research to explore the molecular mechanism of colorectal cancer occurrence and development and find potential therapeutic targets through self-gene editing and modification research,or in combination with Air-liquid interface culture and microfluidic technology research.This article reviews the current status and progress of colorectal cancer organoids in clinical application and basic research.

关 键 词：类器官 结直肠癌 转化医学 肿瘤微环境 类器官芯片 

分 类 号：R735.34[医药卫生—肿瘤]