缓激肽受体B1R对SD大鼠阴茎勃起功能影响的实验研究  

Experimental Study on the Effect of Bradykinin Receptor B1R on the Erectile Function of the SD Rat

作　　者：斯依提·阿木提 沙丽帕·艾尼瓦尔 阿地力江·伊明 王冰华 薛志琴 刘文娟 Siyiti·Amuti;Shalipa·Ainiwaer;Adiljiang·Yiming;WANG Bing-hua;XUE Zhi-qin;LIU Wen-juan(Department of Human Anatomy,College of Basic Medical Sciences,Xinjiang Medical University,Urumqi,Xinjiang,830054,China)

机构地区：[1]新疆医科大学基础医学院人体解剖学教研室,新疆乌鲁木齐830054

出　　处：《现代生物医学进展》2022年第22期4219-4223,共5页Progress in Modern Biomedicine

基　　金：新疆维吾尔自治区科学技术厅青年基金项目(2019D01C198)。

摘　　要：目的:研究缓激肽受体B1R对大鼠阴茎勃起功能的影响。方法:通过腹腔注射B1受体激动剂[Des-Arg9]-Bradykinin与B1受体抑制剂Lys-(des-Arg9,Leu8)-Bradykinin,观察各组大鼠阴茎勃起功能,通过HE染色和Masson染色观察大鼠阴茎组织形态变化及纤维化水平的变化,通过Western-blot检测大鼠阴茎组织TGF-β1、TNF-α与IL-6等炎症因子的表达情况。结果:(1)B1受体激动剂显著抑制大鼠阴茎勃起功能,并升高阴茎胶原纤维/肌原纤维比值;而B1受体抑制剂显著提升大鼠阴茎勃起功能,并降低阴茎胶原纤维/肌原纤维比值;(2)B1受体激动剂显著升高大鼠阴茎TGF-β1、TNF-α与IL-6蛋白表达水平,而B1受体抑制剂降低大鼠阴茎TGF-β1、TNF-α与IL-6蛋白表达水平。结论:B1受体可能通过炎症因子、阴茎组织纤维化影响阴茎勃起功能。Objective:To investigate the effect of bradykinin receptor B1R on the erectile function of the rat penis.Methods:By intraperitoneal injection of B1 receptor agonist[Des-Arg9]-Bradykinin and B1 receptor inhibitor Lys-(des-Arg9,Leu8)-Bradykinin,The penile erectile function of rats in each group,the morphological changes and fibrosis level of rat penile tissues were observed,and the expression of TGF-β1,TNF-αand IL-6 were detected.Results:(1)B1 agonists significantly inhibited the erectile function and increased the penile collagen fiber/myogenic fiber ratio,while B1 receptor inhibitors significantly enhanced the erectile function and decreased the penile collagen fiber/myogenic fiber ratio;(2)B1 agonists significantly increased the expression levels of TGF-β1,TNF-αand IL-6 in the rat penis,while B1 receptor inhibitors decreased TGF-β1,TNF-αand IL-6 protein expression levels in the rat penis.Conclusions:B1receptor can affect penile tissue fibrosis and ultimately penile erectile function through inflammatory factors and other pathways.

关 键 词：激肽-激肽释放酶系统 缓激肽受体B1 勃起功能障碍 炎症 

分 类 号：R-33[医药卫生] R697.1[生物学—生理学] Q492.4