Reduced neutralization of SARS-CoV-2 B.1.617 variant by convalescent and vaccinated sera  

作　　者：Jie Hu Xiao-yu Wei Jin Xiang Pai Peng Feng-li Xu Kang Wu Fei-yang Luo Ai-shun Jin Liang Fang Bei-zhong Liu Kai Wang Ni Tang Ai-Long Huang 

机构地区：[1]Key Laboratory of Molecular Biology for Infectious Diseases(Ministry of Education),Institute for Viral Hepatitis,Department of Infectious Diseases,The Second Affiliated Hospital,Chongqing Medical University,Chongqing 400010,PR China [2]Yong-Chuan Hospital,Chongqing Medical University,Chongqing 402177,PR China [3]Department of Immunology,College of Basic Medicine,Chongqing Medical University,Chongqing 400016,PR China

出　　处：《Genes & Diseases》2022年第5期1290-1300,共11页基因与疾病（英文）

基　　金：supported by the National Natural Science Foundation of China(No.U20A20392);the 111 Project(No.D20028);Open Research Fund Program of the Key Laboratory of Molecular Biology for Infectious Diseases,China(No.CQMU202102 and CQMU202105);The Science and Technology Research Program of Chongqing Municipal Education Commission,China(No.KJZD-M202000401);The Natural Science Foundation Project of Chongqing,China(No.cstc2019jscx-dxwtBX0019);The Emergency Project from the Science&Technology Commission of Chongqing,China(No.cstc2020jscx-fyzx0053 and cstc2020jscx-dxwtB0050);Kuanren Talents Program of the second affiliated hospital of Chongqing Medical University,the Emergency Project for Novel Coronavirus Pneumonia from the Chongqing Medical University,China(No.CQMUNCP0302);China Postdoctoral Science Foundation,China(No.2021M693924);Chongqing Postdoctoral Science Special Foundation,China(No.2010010005216630).

摘　　要：Coronavirus disease 2019(COVID-19)is caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The Spike protein that mediates coronavirus entry into host cells is a major target for COVID-19 vaccines and antibody therapeutics.However,multiple variants of SARS-CoV-2 have emerged,which may potentially compromise vaccine effectiveness.Using a pseudovirus-based assay,we evaluated SARS-CoV-2 cell entry mediated by the viral Spike B.1.617 and B.1.1.7 variants.We also compared the neutralization ability of monoclonal antibodies from convalescent sera and neutralizing antibodies(NAbs)elicited by CoronaVac(inactivated vaccine)and ZF2001(RBD-subunit vaccine)against B.1.617 and B.1.1.7 variants.Our results showed that,compared to D614G and B.1.1.7 variants,B.1.617 shows enhanced viral entry and membrane fusion,as well as more resistant to antibody neutralization.These findings have important implications for understanding viral infectivity and for immunization policy against SARS-CoV-2 variants.

关 键 词：CORONAVIRUS Immune escape Mutation Neutralizing antibodies SARS-CoV-2 VACCINE Viral entry 

分 类 号：R563.1[医药卫生—呼吸系统] R186[医药卫生—内科学]