CCNB3和BCOR在BCOR-CCNB3肉瘤中的表达及诊断价值  

The diagnostic value of CCNB3 and BCOR expression in BCOR-CCNB3 sarcoma

作　　者：李兰[1] 张铭[1] 孙晓淇[1] 张婷婷[1] 丁宜[1] Li Lan;Zhang Ming;Sun Xiaoqi;Zhang Tingting;Ding Yi(Department of Pathology,Beijing Jishuitan Hospital,Fourth Medical College of Peking University,Beijing 100035,China)

机构地区：[1]北京积水潭医院,北京大学第四临床医学院病理科,北京100035

出　　处：《中华病理学杂志》2022年第12期1235-1239,共5页Chinese Journal of Pathology

基　　金：北京市科技计划(Z171100001017134);北京积水潭医院“学科新星”计划(XKXX201811)。

摘　　要：目的探讨CCNB3和BCOR在BCOR-CCNB3肉瘤(BCOR-CCNB3 sarcoma,BCS)中的表达情况及诊断价值。方法收集北京积水潭医院病理科2014年1月至2021年10月诊断的15例经荧光原位杂交(FISH)和/或逆转录-聚合酶链反应(RT-PCR)证实的BCS,采用免疫组织化学EnVision法检测CCNB3和BCOR在15例BCS及65例其他类型软组织肿瘤(54例尤因肉瘤、5例CIC重排肉瘤、4例滑膜肉瘤、1例间叶性软骨肉瘤以及1例软组织透明细胞肉瘤)中的表达情况。结果15例BCS中,14例(14/15)肿瘤细胞呈CCNB3阳性;而在65例非BCS肿瘤中均为阴性。11例(11/14)BCS肿瘤细胞呈BCOR阳性,而在65例非BCS肿瘤中仅有7例(7/65,10.8%)阳性(尤因肉瘤5例、滑膜肉瘤1例、间叶性软骨肉瘤1例)。CCNB3诊断BCS的灵敏度和特异度分别为93.3%和100%;而BCOR的灵敏度和特异度分别为78.6%和89.2%。结论CCNB3是诊断BCS灵敏度和特异度均较高的标志物,可用于BCS初筛。Objective To investigate the diagnostic value of expression of CCNB3 and BCOR in BCOR-CCNB3 sarcoma(BCS).Methods Fifteen cases of BCS confirmed by fluorescence in situ hybridization(FISH)and/or reverse transcription-polymerase chain reaction(RT-PCR)from January 2014 to October 2021 at Beijing Jishuitan Hospital were collected.Immunohistochemical EnVision method was used to detect the expression of CCNB3 and BCOR in 15 cases of BCS and in 65 non-BCS tumors(54 cases of Ewing′s sarcoma,5 cases of CIC rearranged sarcoma,4 cases of synovial sarcoma,1 case of mesenchymal chondrosarcoma and 1 case of soft tissue clear cell sarcoma).Results Immunohistochemical staining for CCNB3 revealed strongly diffuse nuclear staining in 14 of 15(14/15)BCS cases,whereas none of the 65 non-BCS tumors showed any staining.Immunohistochemical staining for BCOR showed strongly diffuse nuclear staining in 11(11/14)BCS cases;seven of the 65(7/65,10.8%)non-BCS tumors showed variable staining(five cases of Ewing sarcoma,one cases of synovial sarcoma,and one case of mesenchymal chondrosarcoma).The sensitivity and specificity of CCNB3 in diagnosing BCS were 93.3%and 100%and these of BCOR were 78.6%and 89.2%,respectively.Conclusions CCNB3 is a highly sensitive and specific marker for BCS.The antibody may help screening BCS.

关 键 词：软组织肿瘤 免疫组织化学 BCOR-CCNB3肉瘤 

分 类 号：R738.6[医药卫生—肿瘤]