高脂日粮中添加燕麦纤维对大鼠肠道菌群及胰岛素抵抗的影响  

Effect of oat fiber added to high fat diet on intestinal flora and insulin resistance in rats

作　　者：秦廷洋 龙红 代军 张仕斌 QIN Ting-yang;LONG Hong;DAI Jun;ZHANG Shi-bin

机构地区：[1]遵义医科大学实验动物中心,贵州遵义563000

出　　处：《饲料研究》2022年第21期82-86,共5页Feed Research

基　　金：遵义市红花岗区科学技术项目(项目编号:遵红科合社字(2019) 31号);遵义医科大学硕士启动基金项目(项目编号:F-834)。

摘　　要：试验旨在探究高脂日粮条件下,添加燕麦膳食纤维对大鼠肠道微生物和胰岛素抵抗影响和作用机制。选取18只250~260 g雌性大鼠,随机分为2组,每组3个重复,每个重复3只鼠。HFD组大鼠饲喂高脂饲料,F-HFD组大鼠饲喂高脂饲料添加5%燕麦膳食纤维。试验期8周。结果显示:F-HFD组大鼠的血糖值显著低于HFD组(P<0.05),F-HFD组大鼠胰岛素抵抗指数(HOMA-IR)显著低于HFD组(P<0.05)。在糖耐受试验中,注射葡萄糖15 min时,F-HFD组大鼠血糖值显著低于HFD组(P<0.05),F-HFD组大鼠曲下面积(AUC)显著低于F-HFD组(P<0.05)。在肠道菌群种群分析中,与HFD组相比,F-HFD组大鼠的颤螺菌属(Oscillospira)相对丰度明显下调,拟杆菌属(Bacteroides)和粪球菌属(Coprococcus)的相对丰度明显上调。相关性分析表明,Oscillospira与脂多糖(LPS)、HOMA-IR和AUC呈显著正相关,Bacteroides与Coprococcus与LPS、HOMA-IR和AUC呈显著负相关。研究表明,膳食纤维可能通过调节高糖高脂日粮组大鼠肠道Oscillospira、Bacteroides与Coprococcus的相对丰度,进而改变机体内LPS含量,改善高脂高糖膳食所造成的胰岛素抵抗状态。The study was to investigate the effect and possible mechanism of oat dietary fiber on intestinal microbiota and insulin resistance in rats fed a high-fat diet. Eighteen 250~260 g female rats were randomly divided into two groups, with three replicates in each group, and three rat in each replicate. Rats in HFD group were fed with a high-fat diet, and rats in F-HFD group were fed with high-fat diet supplemented with 5% oat dietary fiber. The experiment lasted for 8 w. The results showed that the blood glucose value of rats in F-HFD group was significantly lower than that in HFD group(P<0.05), and HOMA-IR index of rats in F-HFD group was significantly lower than that in basic HFD group(P<0.05). In glucose tolerance test, at 15 min after glucose injection, the blood glucose value of rats in F-HFD group was significantly lower than that in HFD group(P<0.05), and the area under curvature(AUC) of rats in F-HFD group was significantly lower than that in HFD group(P<0.05). In the intestinal microbiota population analysis, compared with HFD group, the relative abundance of Oscillospira was significantly down-regulated and the relative abundance of Bacteroides and Coprococcus were significantly up-regulated in F-HFD group. Correlation analysis showed that Oscillospira was significantly positively correlated with LPS, HOMA-IR and AUC, while Bacteroides and Coprococcus were significantly negatively correlated with LPS, HOMA-IR and AUC. The study indicates that dietary fiber may change LPS content and improve insulin resistance of rats fed high-glucose and high-fat diet by regulating the relative abundance of Oscillospira,Bacteroides and Coprococcus in intestinal tract.

关 键 词：大鼠 膳食纤维 高脂日粮 胰岛素抵抗 肠道微生物 

分 类 号：S816[农业科学—饲料科学]