血清miR-30c-2-3p与卵巢癌组织内质网应激相关蛋白表达及患者预后的相关性  

Correlation between serum miR-30c-2-3p and expression of endoplasmic reticulum stress related protein in ovarian cancer tissues,prognosis of patients

作　　者：张纪妍 曾赛田[1] 奚杰[1] ZHANG Jiyan;ZENG Saitian;XI Jie(Department of Gynecology,Cangzhou Central Hospital,Cangzhou 061000,China)

机构地区：[1]沧州市中心医院妇科,河北沧州061000

出　　处：《东南大学学报（医学版）》2022年第5期681-687,共7页Journal of Southeast University(Medical Science Edition)

摘　　要：目的:分析血清miR-30c-2-3p与卵巢癌(OC)组织内质网应激(ERS)相关蛋白表达、患者临床特征及预后的关系。方法:招募2018年1月至2020年12月期间70例于我院接受治疗的OC患者(OC组),并纳入同期100例健康志愿者作为对照组。采用蛋白质印迹法分析OC组织C/EBP同源蛋白(CHOP)、葡萄糖调节蛋白78(BIP)的表达情况。使用定量实时聚合酶链反应检测血清miR-30c-2-3p表达。随访记录患者的无病生存期(DFS)。结果:OC组患者基线血清miR-30c-2-3p表达显著低于对照组受试者(0.77±0.25 vs.1.15±0.39,t=7.869,P<0.001)。经受试者工作特征曲线分析,基线血清miR-30c-2-3p表达诊断OC的曲线下面积为0.785(95%CI 0.718~0.852)。基线血清miR-30c-2-3p表达≤0.703的患者FIGO分期Ⅲ~Ⅳ期概率更大,肿瘤直径也更大(P<0.05)。基线血清miR-30c-2-3p表达水平与OC组织CHOP表达呈正相关(r=0.483,P<0.001),与BIP表达呈显著负相关(r=-0.240,P=0.045)。经多元线性回归分析,OC组织中CHOP(t=4.080,P<0.001)、BIP(t=-2.048,P=0.045)都是影响基线血清miR-30c-2-3p表达水平的独立因子。绘制Kaplan-Meier生存曲线,基线血清miR-30c-2-3p低表达OC患者中位DFS显著短于高表达者(χ^(2)=7.515,P=0.006)。经多因素COX回归分析显示,基线血清miR-30c-2-3p低表达为OC患者术后复发的独立临床因素(OR=0.399,95%CI 0.201~0.791,P=0.009)。结论:血清miR-30c-2-3p在OC患者中多呈低表达,并可能通过调节ERS影响患者预后。因此,血清miR-30c-2-3p表达对OC患者术后复发具有预测价值。Objective:To analyze the relationship between serum miR-30c-2-3p and tissue expressions of endoplasmic reticulum stress(ERS)related protein,clinical features and prognosis of ovarian cancer(OC).Methods:70 OC patients(OC group)who underwent treatment in our hospital from January 2018 to December 2020 were recruited,and a total 100 healthy volunteers during the same period were included as the control group.Western blot was used to analyze the expressions of C/EBP homologous protein(CHOP)and glucose regulated protein 78(BIP)in OC tissues.Serum miR-30c-2-3p expression was detected by quantitative real-time polymerase chain reaction.Patients disease-free survival(DFS)was recorded during follow-up.Results:The baseline serum miR-30c-2-3p expression of OC group was significantly lower than that of the control group(0.77±0.25 vs.1.15±0.39,t=7.869,P<0.001).According to the analysis of the receiver operating characteristic curve,the area under the curve of baseline serum miR-30C-2-3p expression for OC diagnosis was 0.785(95%CI 0.718-0.852).Patients with baseline serum miR-30c-2-3p≤0.703 had higher probability of FIGOⅢtoⅣstaging and larger tumor diameter(P<0.05).The expression level of miR-30c-2-3p in baseline serum was positively correlated with the expression of CHOP in OC tissue(r=0.483,P<0.001),and significantly negatively correlated with the expression of BIP(r=-0.240,P=0.045).By multiple linear regression analysis,CHOP(t=4.080,P<0.001)and BIP(t=-2.048,P=0.045)in OC tissues were independent factors that affected the expression level of miR-30c-2-3p in baseline serum.Kaplan-Meier survival curve was plotted,and the median DFS of OC patients with low baseline serum miR-30c-2-3p expression was significantly shorter than that of OC patients with high expression(χ^(2)=7.515,P=0.006).Multivariate COX regression analysis showed that the low baseline serum miR-30C-2-3p expression was an independent clinical factor for postoperative recurrence in OC patients(OR=0.399,95%CI 0.201-0.791,P=0.009).Conclusion:Serum miR-30c-2

关 键 词：卵巢癌 miR-30c-2-3p 内质网应激 复发 预后 

分 类 号：R737.31[医药卫生—肿瘤]