miR-18b-5p调控WWOX对胶质瘤细胞增殖、凋亡的影响  

miR-18b-5p regulates proliferation and apoptosis of glioma cells by targeting WW domam-containing oxidoreductase

作　　者：苏保寿 薛治乾 余鹏飞 吴益敏 喻闻庆 SU Bao-shou;XUE Zhi-qian;YU Peng-fei;WU Yi-min;YU Wen-qing(Department of Neurosurgery,Danzhou People′s Hospital,Danzhou Hainan 571799,China;Department of Pathology,Danzhou People′s Hospital,Danzhou Hainan 571799,China)

机构地区：[1]海南省儋州市人民医院神经外科,571799 [2]海南省儋州市人民医院病理科,571799

出　　处：《蚌埠医学院学报》2022年第12期1633-1638,共6页Journal of Bengbu Medical College

基　　金：海南省卫生计生行业科研项目(18A200157)。

摘　　要：目的:研究miR-18b-5p对胶质瘤U251细胞增殖、凋亡的影响和潜在的分子机制。方法:qRT-PCR检测癌旁组织和胶质瘤组织中miR-18b-5p的表达,MTT法和流式细胞术检测胶质瘤U251细胞增殖和凋亡率,Western blotting检测包含WW域的氧化还原酶基因(WWOX)、Cyclin D1、p21、Bax和Bcl-2蛋白表达,双荧光素酶报告系统验证miR-18b-5p和WWOX的调控关系。结果:与癌旁组织相比,胶质瘤组织中miR-18b-5p的表达量升高(P<0.01)。抑制miR-18b-5p表达可以抑制胶质瘤U251细胞增殖并促进细胞凋亡;miR-18b-5p靶向负调控WWOX的表达;过表达WWOX可抑制U251细胞增殖并诱导细胞凋亡;抑制WWOX表达逆转了抑制miR-18b-5p表达对U251细胞增殖、凋亡的影响(P<0.01)。结论:miR-18b-5p可能通过靶向调控WWOX表达抑制胶质瘤U251细胞增殖并诱导细胞凋亡,miR-18b-5p是胶质瘤潜在的分子靶点。Objective:To investigate the effects of miR-18b-5p on the proliferation and apoptosis of glioma U251 cells and the underlying mechanism.Methods:The expression levels of miR-18b-5p in glioma tissues and adjacent tissues were measured by quantitative real-time polymerase chain reaction(qRT-PCR).The proliferation and apoptotic rate of U251 cells were determined by MTT assay and flow cytometry.The expressions of WWOX,Cyclin D1,p21,Bax and Bcl-2 proteins were detected by Western blotting.The relationship between miR-18b-5p and WWOX was verified by dual-luciferase reporter assay system.Results:Compared with the adjacent tissues,the expression of miR-18b-5p in glioma tissues was significantly increased(P<0.01).Inhibition of miR-18b-5p inhibited the proliferation and induced apoptosis of U251 cells.MiR-18b-5p targeted and negatively regulated the expression of WWOX.Over-expression of WWOX inhibited the proliferation and induced apoptosis of U251 cells.Inhibition of WWOX reversed the effects of miR-18b-5p inhibition on cell proliferation and apoptosis of U251 cells(P<0.01).Conclusions:MiR-18b-5p may inhibit the proliferation and induce apoptosis of glioma U251 cells by targeting WWOX.MiR-18b-5p is a potential molecular target of glioma.

关 键 词：胶质瘤 U251细胞 miR-18b-5p 包含WW域的氧化还原酶基因 

分 类 号：R739.41[医药卫生—肿瘤]