长链非编码RNA LINP1抑制DNA损伤促进非小细胞肺癌电离辐射抗性  

Long non-coding RNA LINP1 inhibits the DNA damage induced by ionizing radiation and promote radioresistance in non-small cell lung cancer

作　　者：张春婷 梁枭婷 王乐 周良 ZHANG Chunting;LIANG Xiaoting;WANG Le;ZHOU Liang(Department of Toxicology,School of Public Health,Southern Medical University(Guangdong Provincial Key Laboratory of Tropical Disease Research),Guangzhou 510515,China)

机构地区：[1]南方医科大学公共卫生学院(广东省热带病研究重点实验室)毒理学系,广州510515

出　　处：《实用医学杂志》2022年第23期2908-2913,共6页The Journal of Practical Medicine

基　　金：2021年广东省自然科学基金面上项目(编号:2021A1515010855);2021年南方医科大学大学生创新创业训练计划项目(编号:202112121270)。

摘　　要：目的探索长链非编码RNA LINP1对电离辐射下非小细胞肺癌DNA损伤效应的影响,为非小细胞肺癌的肿瘤治疗提供相关的新思路。方法使用siRNA在非小细胞肺癌细胞系H1299细胞中敲减LINP1,8.0 Gy电离辐射照射后,采用CCK-8增殖实验和克隆形成实验检测肺癌细胞的增殖和克隆形成能力;照射后4 h采用彗星电泳和Western blot检测H1299细胞中DNA损伤效应。结果电离辐射照射后,H1299细胞中LINP1明显上调(P<0.001)。与对照相比,8.0 Gy电离辐射照射后其增殖和克隆形成能力显著下降(P<0.001),DNA损伤明显增加(P<0.001)。沉默LINP1后,在电离辐射条件下,敲减组与对照相比增殖能力、克隆形成能力明显下降(P<0.01),DNA损伤明显增加(P<0.001)。结论LINP1可抑制电离辐射下非小细胞肺癌细胞增殖能力的下降和DNA损伤效应从而增强电离辐射抗性。Objective To explore the effect of long non-coding RNA LINP1 on ionizing radiation-induced DNA damage in non-small cell lung cancer(NSCLC)and provide theoretical basis for novel combination treatment option.Methods H1299 NSCLC cells were transfected with siRNAs.CCK-8 assays and colony formation assays were used to check for H1299 cell growth after 8.0 Gy of ionizing radiation.To find the DNA damage in H1299cells,Western blot and comet assay were used.Results After receiving ionizing radiation(IR),LINP1 was significantly(P<0.001)upregulated in H1299 cells.Compared with the control,the proliferation capacity and clonal formation ability decreased(P<0.001),while the DNA damage(P<0.001)was increased significantly(P<0.001).Under 8.0 Gy IR treatment,LINP1 knockdown significantly reduced H1299 cell proliferation and clonal formation ability(P<0.01),as well as DNA damage repair ability(P<0.001).Conclusions LINP1 inhibits proliferation and increases IR-induced DNA damage in NSCLC cells,while also promoting radioresistance.

关 键 词：长链非编码RNA LINP1 非小细胞肺癌 DNA损伤 电离辐射抗性 

分 类 号：R734.2[医药卫生—肿瘤]