年轻Sca-1骨髓干细胞对年老小鼠心外膜细胞衰老的调控作用及机制研究  

Regulating role of young Sca-1 bone marrow stem cell on epicardial cell senescence and its mechanism

作　　者：董珺[1] 吕饶 高芳[2] 于佳迪 李柳蓁 湛楚蓝 李月亮 刘慰华[1] 黎佼[1] DONG Jun;Lü Rao;GAO Fang;YU Jiadi;LI Liuzhen;ZHAN Chulan;LI Yueliang;LIU Weihua;LI Jiao(The Second Affiliated Hospital of Guangzhou Medical University,Guangzhou 510260,China;不详)

机构地区：[1]广州医科大学附属第二医院,广州510260 [2]山东省滨州市人民医院,山东滨州256600 [3]广州医科大学附属第六医院,广东清远511518

出　　处：《实用医学杂志》2022年第23期2914-2918,共5页The Journal of Practical Medicine

基　　金：广州市科技计划项目(编号:202002030336);广州医科大学附属第六医院,清远市人民医院开放课题基金项目(编号:202201-304);广东省基础与应用基础研究基金(编号:2019A1515011214,2021A1515011364);广州医科大学附属第二医院临床研究项目(编号:2021-LCYJ-DZX-03,2022-LCYJ-YYDZX-03);广州医科大学2022年度学生创新能力提升计划项目(编号:广医大发[2022]66号)。

摘　　要：目的探讨年轻Sca-1骨髓干细胞对年老小鼠心外膜细胞衰老的调控作用及机制。方法将年轻和年老小鼠Sca-1骨髓干细胞分别与年老小鼠心外膜细胞共培养(分为Y Sca-1细胞组与O Sca-1细胞组)。通过细胞染色、基因及蛋白检测,探讨Sca-1骨髓干细胞对年老小鼠心外膜细胞衰老的影响。结果与O Sca-1细胞组相比,Y Sca-1细胞组可明显减少心外膜细胞衰老:P16免疫荧光染色阳性细胞比例下降;P27基因及蛋白表达减少;Sirt 1基因及蛋白表达增加(P<0.05)。分子机制研究发现,与O Sca-1细胞相比,Y Sca-1细胞可旁分泌更多的GDNF(P<0.05)。中和GDNF后,使Y Sca-1细胞失去减少年老心外膜细胞衰老的作用。结论年轻Sca1骨髓干细胞可通过旁分泌GDNF减少年老心外膜细胞衰老。Objective To study the regulating effect of young Sca-1 bone marrow stem cell on epicardial cell senescence and its mechanism.Methods Young(Y)and old(O)Sca-1 bone marrow cell was co-cultured with old epicardial cell under hypoxia condition,respectively.The effect of Sca-1 bone marrow cell on epicardial cell senescence was detected by immunofluorescent staining,qRT-PCR,and western blot,respectively.Results Compared to O Sca-1 bone marrow cell,Y Sca-1 cell decreased epicardial cell senescence,with decreased senescent cell rate,decreased P27 gene and protein expression,and increased Sirt 1 gene and protein expression(P<0.05).More GDNF(Glial cell line-Derived Neurotrophic Factor)was found in Y Sca-1 cell group when compared to that in O Sca-1 cell group(P<0.05).The effect of Y Sca-1 cell on old epicardial cell senescence was lost when GDNF expression was blocked.Conclusion Young Sca-1 bone marrow stem cell decreases old epicardial cell senescence by GDNF.

关 键 词：干细胞 心外膜细胞 衰老 旁分泌 生长因子 

分 类 号：R363.1[医药卫生—病理学]