急性脑梗死患者静脉溶栓前后闭锁蛋白、甲基CpG结合蛋白2、钙调蛋白表达与功能结局的关系  

The Relationship between the Expression of Occludin,Methyl-CpG-binding Protein 2 and Calmodulin and Functional Outcomes before and after Intravenous ThromBolysis in Patients with Acute Cerebral Infarction

作　　者：何健萍 彭国斌 HE Jianping;PENG Guobin(Department of Neurology,the First People’s Hospital of Zhoukou City,He’nan Province 466700)

机构地区：[1]河南省周口市第一人民医院神经内科,466700

出　　处：《医学理论与实践》2023年第1期10-13,共4页The Journal of Medical Theory and Practice

摘　　要：目的:分析急性脑梗死(ACI)患者静脉溶栓前后闭锁蛋白(Occludin)、甲基CpG结合蛋白2(MECP2)、钙调蛋白(CAM)表达与功能结局的关系。方法:选取2020年1月-2022年1月我院收治的ACI患者134例作为ACI组,同期进行健康体检的健康志愿者50例作为NC组。使用酶标仪测定Occludin、MECP2、CAM水平,在ACI患者静脉溶栓后90d时,使用日常生活活动能力量表评价功能结局,根据ACI患者静脉溶栓后功能结局分为结局不良组、结局良好组两个亚组。分析ACI患者静脉溶栓前后Occludin、MECP2、CAM表达变化及与功能结局的关系。结果:ACI组Occludin水平低于NC组,MECP2、CAM水平高于NC组,差异具有统计学意义(P<0.05)。ACI患者溶栓后90d Occludin水平高于溶栓前,MECP2、CAM水平低于溶栓前,差异具有统计学意义(P<0.05)。结局不良组Occludin水平低于结局良好组,MECP2、CAM水平高于结局良好组,差异具有统计学意义(P<0.05)。由Pearson相关性分析发现,Occludin、MECP2、CAM与ACI患者静脉溶栓后功能结局相关(均P<0.05)。ROC曲线显示,相比于Occludin、MECP2、CAM单项预测,三项联合预测效能最佳,三项联合预测的AUC为0.912(95%CI=0.828~1.036),敏感度为89.00%,特异度为81.50%,P<0.001。结论:ACI患者Occludin水平降低,MECP2、CAM水平升高,静脉溶栓可改善Occludin、MECP2、CAM水平,而溶栓功能结局不良与Occludin、MECP2、CAM异常有关。Objective:To analyze the relationship between the expressions of Occludin,Methyl-CpG-binding protein 2(MECP2)and Calmodulin(CAM)and functional outcomes before and after intravenous thrombolysis in patients with acute cerebral infarction(ACI).Methods:A total of 134 ACI patients admitted to our hospital from January 2020 to January 2022 were selected as the ACI group,and 50 healthy volunteers who underwent physical examinations during the same period were selected as the NC group.The levels of Occludin,MECP2 and CAM were measured by microplate reader.At 90 days after intravenous thrombolysis in ACI patients,the activity of daily living scale was used to evaluate the functional outcome.According to ACI The functional outcomes of patients after intravenous thrombolysis were divided into two subgroups:poor outcome group and good outcome group.The expression changes of Occludin,MECP2 and CAM in ACI patients before and after intravenous thrombolysis and their relationship with functional outcomes were analyzed.Results:The level of Occludin in the ACI group was lower than that in the NC group,and the levels of MECP2 and CAM were higher than those in the NC group,and the differences were statistically significant(P<0.05).The levels of Occludin 90 days after thrombolysis in ACI patients were higher than those before thrombolysis,and the levels of MECP2 and CAM were lower than those before thrombolysis,and the differences were statistically significant(P<0.05).The level of Occludin in the poor outcome group was lower than that in the good outcome group,and the levels of MECP2 and CAM were higher than those in the good outcome group,and the difference was statistically significant(P<0.05).The Pearson correlation analysis found that Occludin,MECP2,and CAM were associated with the functional outcome of patients with ACI after intravenous thrombolysis(all P<0.05).The ROC curve showed that the three combined prediction efficiency was the best compared to Occludin,MECP2,and CAM.They predicted AUC of 0.912(95%CI=0.828~1.036),sensi

关 键 词：急性脑梗死 闭锁蛋白 甲基CpG结合蛋白2 钙调蛋白 功能结局 

分 类 号：R743.3[医药卫生—神经病学与精神病学]