鲜铁皮石斛对肺腺癌A549细胞的作用及其可能机制  

Effect of fresh Dendrobium candidum on pulmonary adenocarcinoma A 549 cells and its possible mechanism

作　　者：林岳岩 张秀玲 韦明[1] 黄敏[1] 张沥仁 朱莉莉 覃议贤 张锡流[1] LIN Yue-yan;ZHANG Xiu-ling;WEI Ming;HUANG Min;ZHANG Li-ren;ZHU Li-li;QIN Yi-xian;ZHANG Xi-liu(Department of Pathology,the First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,Guangxi,China)

机构地区：[1]广西中医药大学附属第一医院病理科,广西南宁市530023

出　　处：《广西医学》2022年第22期2645-2648,2683,共5页Guangxi Medical Journal

基　　金：广西自然科学基金(2017GXNSFAA198175)。

摘　　要：目的探讨鲜铁皮石斛对人肺腺癌A549细胞增殖、迁移能力的影响及其可能机制。方法(1)采用0.5 mg/mL、1.0 mg/mL、1.5 mg/mL、2.0 mg/mL的鲜铁皮石斛(分别设为0.5 mg/mL组、1.0 mg/mL组、1.5 mg/mL组、2.0 mg/mL组)干预A549细胞24 h、48 h后,应用CCK-8法测定A549细胞的增殖抑制率及半数抑制浓度(IC_(50))。(2)将A549细胞分为空白组和鲜铁皮石斛组,其中鲜铁皮石斛组的鲜铁皮石斛干预浓度为IC_(50),干预12 h、24 h后分别进行细胞划痕实验,计算A549细胞的愈合率。(3)将细胞分为低浓度组、中浓度组、高浓度组和空白组,分别给予0.5倍IC_(50)、1.0倍IC_(50)、2.0倍IC_(50)、0 mg/mL鲜铁皮石斛干预。24 h后通过Hoechst 33258荧光染色观察细胞凋亡情况。结果(1)干预24 h后,1.5 mg/mL组、2.0 mg/mL组A549细胞的增殖抑制率高于0.5 mg/mL组(P<0.05),且1.0 mg/mL组、1.5 mg/mL组、2.0 mg/mL组A549细胞的增殖抑制率依次升高(均P<0.05)。干预48 h后,0.5 mg/mL组、1.0 mg/mL组、1.5 mg/mL组、2.0 mg/mL组A549细胞的增殖抑制率依次升高(均P<0.05)。除0.5 mg/mL组外,其余浓度组中干预48 h后的A549细胞增殖抑制率均高于干预24 h后(均P<0.05)。(2)干预12 h、24 h后鲜铁皮石斛组A549细胞的愈合率均高于空白组(均P<0.05)。(3)干预24 h后,低、中、高浓度组A549细胞出现较为明显的凋亡形态学特征,且凋亡细胞数量随浓度增加呈现增多趋势。结论鲜铁皮石斛能够抑制肺腺癌A549细胞增殖及迁移能力,这一作用可能通过促进细胞凋亡而实现。Objective To investigate the effect of fresh Dendrobium candidum on proliferation and migration of human pulmonary adenocarcinoma A549 cells and its possible mechanism.Methods(1)Fresh Dendrobium candidum of 0.5 mg/mL,1.0 mg/mL,1.5 mg/mL,and 2.0 mg/mL(set as the 0.5 mg/mL group,the 1.0 mg/mL group,the 1.5 mg/mL group,or the 2.0 mg/mL group,respectively)were employed to intervene A549 cells for 24 and 48 hours.The proliferation inhibition rate and half maximal inhibitory concentration(IC_(50))of A549 cells were measured by the CCK-8 method.(2)A549 cells were assigned to blank group or fresh Dendrobium candidum group,therein the Dendrobium candidum intervention concentration of the Dendrobium candidum group was IC_(50).After 12 and 24 hours of intervention,the wounding healing assay was performed,and the healing rate of A549 cells was calculate,respectively.(3)Cells were assigned to low-,medium-,and high-concentration groups or blank group,and fresh Dendrobium candidum intervention was given with 0.5 times of IC_(50),1.0 times of IC_(50),2.0 times of IC_(50),and 0 mg/mL,respectively.The cell apoptosis was observed by the Hoechst 33258 fluorescent staining after 24 hours.Results(1)After 24 hours of intervention,the proliferation inhibition rate of A549 cells in the 1.5 mg/mL group and the 2.0 mg/mL group was higher than that in the 0.5 mg/mL group(P<0.05),and the proliferation inhibition rate of A549 cells in the 1.0 mg/mL group,the 1.5 mg/mL group,and the 2.0 mg/mL group elevated successively(all P<0.05).After 48 hours of intervention,the proliferation inhibition rate of A549 cells in the following groups elevated successively:the 0.5 mg/mL group,the 1.0 mg/mL group,the 1.5 mg/mL group,and the 2.0 mg/mL group(all P<0.05).Except for the 0.5 mg/mL group,the remaining concentration groups exhibited a higher proliferation inhibition rate of A549 cells after 48 hours of intervention as compared with after 24 hours of intervention(all P<0.05).(2)After 12 and 24 hours of intervention,the healing rate of A549 cells in the f

关 键 词：鲜铁皮石斛 肺腺癌 A549细胞 细胞增殖 细胞凋亡 

分 类 号：R734.2[医药卫生—肿瘤] R28[医药卫生—临床医学]