Phylogenomic relationships and species identification of the olive genus Olea (Oleaceae)  

作　　者：Wen-Pan Dong Jia-Hui Sun Yan-Lei Liu Chao Xu Yi-Heng Wang Zhi-Li Suo Shi-Liang Zhou Zhi-Xiang Zhang Jun Wen 

机构地区：[1]Laboratory of Systematic Evolution and Biogeography of Woody Plants,School of Ecology and Nature Conservation,Beijing Forestry University,Beijing100083,China [2]State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China [3]state Key Laboratory of Systematic and Evolutionary Botany,Institute of Botany,The Chinese Academy of Sciences,Beijing 100093,China [4]Department of Botany,National Museum of Natural History,Smithsonian Institution,Washington,DC 20013-7012,USA

出　　处：《Journal of Systematics and Evolution》2022年第6期1263-1280,共18页植物分类学报（英文版）

基　　金：This study was financially supported by the Fundamental Research Funds for the Central Universities(No.BLX201932);National Natural Science Foundation of China(Nos.31770744 and 31620103902);the Fundamental Research Funds for the Central Public Welfare Research Institutes(Nos.ZZXT201901 and ZZXT202003).

摘　　要：The olive genus Olea includes C.30-40 taxa in three subgenera(Olea,Tetrapilus,and Paniculatae)within the family Oleaceae.Historically,the Olea genus was clasifed into four groups that were overall well supported by reconstructed phylogenies,despite incomplete sampling of subgenus Tetrapilus and poor resolution within clades.These analyses also showed that the genus was not monophyletic.Reliable identification of Olea species is important for both their conservation and utilization of this economically important genus.In this study,we used phylogenomic data from genome skimming to resolve relationships within Olea and to identify molecular markers for species identification.We assembled the complete plastomes,and nrDNA of 26 individuals representing 13 species using next generation sequencing and added 18 publicly available accessions of Olea.We also developed nuclear SNPs using the genome skimming data to infer the phylogenetic relationships of Olea.Large-scale phylogenomic analyses of 138 samples of tribe Oleeae supported the polyphyly of Olea,with Olea caudatilimba and Olea subgenus Tetrapilus not sharing their most recent common ancestor with the main Olea clade(subgenus Paniculatae and subgenus Olea).The interspecific phylogenetic resolution was poor owing to a possible rapid radiation.By comparing with the plastome data,we identifed the markers ycflb and psbE petL as the best Olea-specific chloroplast DNA barcodes.Compared with universal barcodes,specific DNA barcodes and super-barcode exhibited higher discriminatory power.Our results demonstrated the power of phylogenomics to improve phylogenetic relationships of intricate groups and provided new insights into barcodes that allow for accurate identification of Olea species.

关 键 词：OLEA PHYLOGENOMICS specific DNA barcode species identification super-barcode 

分 类 号：Q96[生物学—昆虫学]