芦笋皂苷调控circ_0001361/miR-136对结肠癌细胞增殖、凋亡的影响  

Effects of asparagus saponin in regulating circ_0001361/miR-136 on the proliferation and apoptosis of colon cancer cells

作　　者：程琳 张刚[2] CHENG Lin;ZHANG Gang(Department of Digestive System Diseases,Qianjiang Central Hospital,Hubei,Qianjiang 433100,China;不详)

机构地区：[1]湖北省潜江市中心医院消化内科,433100 [2]江汉大学附属医院消化二科

出　　处：《河北医药》2022年第22期3370-3374,共5页Hebei Medical Journal

基　　金：武汉市医学科研项目(编号:WX20C29)。

摘　　要：目的考察芦笋皂苷是否通过调控circ_0001361/miR-136影响结肠癌细胞的增殖、凋亡。方法将结肠癌SW1116细胞分为对照组、芦笋皂苷L、M、H组(芦笋皂苷浓度分别为45 mg/L、90 mg/L、180 mg/L)、si-NC组(转染si-NC)、si-circ_0001361组(转染si-circ_0001361)、芦笋皂苷H+pcDNA组(芦笋皂苷180 mg/L+转染pcDNA)、芦笋皂苷H+pcDNA-circ_0001361组(芦笋皂苷180 mg/L+转染pcDNA-circ_0001361)。采用细胞计数试剂盒-8(CCK-8)法检测各组SW1116细胞的抑制率,流式细胞术检测细胞凋亡,克隆形成实验检测细胞克隆形成,Western blot检测B细胞淋巴瘤/白血病-2(Bcl-2)、Bcl-2相关X蛋白(Bax)表达,qRT-PCR检测circ_0001361和miR-136表达。利用荧光素酶报告基因检测分析circ_0001361和miR-136的靶向关系。结果与对照组比较,芦笋皂苷L组、芦笋皂苷M组、芦笋皂苷H组SW1116细胞的抑制率、凋亡率、Bax蛋白、miR-136表达量逐渐增加,克隆数、Bcl-2蛋白、circ_0001361表达量逐渐减少(P<0.05),且芦笋皂苷L组、芦笋皂苷M组、芦笋皂苷H组3组间比较,差异有统计学意义(均P<0.05)。抑制circ_0001361后,si-circ_0001361组SW1116细胞抑制率、凋亡率、Bax蛋白表达量较si-NC组增加,克隆数、Bcl-2蛋白表达量较si-NC组降低(P<0.05)。circ_0001361靶向调控miR-136的表达。芦笋皂苷H+pcDNA-circ_0001361组SW1116细胞抑制率、凋亡率、Bax蛋白表达量低于芦笋皂苷H+pcDNA组,克隆数、Bcl-2蛋白表达量高于芦笋皂苷H+pcDNA组(P<0.05)。结论芦笋皂苷通过下调circ_0001361靶向miR-136,抑制结肠癌SW1116细胞的增殖和诱导凋亡。Objective To investigate the effects of asparagus saponin in regulating circ_0001361/miR-136 on the proliferation and apoptosis of colon cancer cells.Methods The colon cancer SW1116 cells were divided into control group,asparagus saponin low-dose,medium-dose and high-dose groups(45mg/L,90mg/L,180mg/L,respectively),and si-NC group(transfected with si-NC),si-circ_0001361 group(transfection si-circ_0001361),asparagus saponin H+pcDNA group(asparagus saponin 180mg/L+transfection pcDNA),asparagus saponin H+pcDNA-circ_0001361 group(asparagus saponin 180mg/L+transfection pcDNA-circ_0001361).The cell counting kit-8(CCK-8)was used to detect the inhibition rate of SW1116 cells;flow cytometry detected cell apoptosis;clone formation test detected cell clone formation;Western Blot detected the expressions of B-cell lymphoma/leukemia-2(Bcl-2),Bcl-2 related X protein(Bax);qRT-PCR detected the expressions of circ_0001361 and miR-136.Moreover the targeting relationship between circ_0001361 and miR-136 was analyzed by luciferase reporter gene detection.Results Compared with those in control group,the inhibition rate,apoptosis rate,and the expression le3vels of Bax protein and miR-136 in SW1116 cells in asparagus saponin H,M,L groups were gradually increased,however,the number of clones,the expression levels of Bcl-2 protein and circ_0001361 were gradually decreased(P<0.05),and there were significant differences among the three groups(P<0.05).After inhibiting circ_0001361,the inhibition rate,apoptosis rate and Bax protein expression of SW1116 cells in si-circ_0001361 group were significantly higher than those in si-NC group,however,the number of clones and Bcl-2 protein expression levels were significantly lower than those in si-NC group(P<0.05).The circ_0001361 targetedly regulated the expression of miR-136.Moreover the cell inhibition rate,apoptosis rate,Bax protein expression levels in asparagus saponin H+pcDNA-circ_0001361 group were significantly lower than those in asparagus saponin H+pcDNA group,but,the clone number and Bcl-2

关 键 词：结肠癌 芦笋皂苷 增殖 凋亡 circ_0001361 miR-136 

分 类 号：R733.35[医药卫生—肿瘤]