lncRNA MNX1-AS1在非小细胞肺癌中的表达及调控PI3K/AKT通路对癌细胞生物学行为的影响  

Expression of lncRNA MNX1-AS1 in Non-Small Cell Lung Cancer and the Effect of Regulation of PI3K/AKT Pathway on the Biological Behavior of Cancer Cells

作　　者：刘桂霞[1] 邓科兰[1] 厉银平[1] LIU Guixia;DENG Kelan;LI Yinping(Xiaogan Central Hospital,Hubei Xiaogan 432099,China)

机构地区：[1]湖北省孝感市中心医院呼吸与危重症医学科,湖北孝感432099

出　　处：《河北医学》2023年第1期13-18,共6页Hebei Medicine

基　　金：2020年度孝感市自然科学计划项目,(编号:XGKJ2020010008)。

摘　　要：目的:探讨长链非编码RNA(lncRNA)MNX1-AS1(lncRNA MNX1-AS1)在非小细胞肺癌(NSCLC)中的生物学功能及可能的作用机制。方法:选取2017年6月至2019年3月期间在本院进行手术治疗的75例NSCLC患者癌组织及癌旁组织标本,RT-qPCR法检测标本lncRNA MNX1-AS1表达水平,分析lncRNA MNX1-AS1表达与NSCLC临床病理特征及预后的相关性;体外培养NSCLC细胞系PC-9细胞,分别转染si-MNX1-AS1(si-MNX1-AS1组)、si-NC(si-NC组),MTT法及EdU实验检测各组PC-9细胞增殖;Transwell小室法检测细胞迁移与侵袭;Western blot实验检测PI3K/AKT通路相关蛋白表达。结果:lncRNA MNX1-AS1在NSCLC癌组织与细胞系中表达水平显著升高(P<0.05)。lncRNA MNX1-AS1表达与TNM分期和淋巴转移有关(P<0.05),Kaplan-Meier生存分析显示,lncRNA MNX1-AS1高表达组患者3年累积生存率显著低于低表达组,差异有统计学意义(42.11%vs 81.08%,χ^(2)=11.132,P=0.001);与si-NC组比较,si-MNX1-AS1组PC-9细胞的增殖、迁移与侵袭能力以及p-PI3K、p-AKT、p-mTOR蛋白表达水平显著降低(P<0.05)。结论:lncRNA MNX1-AS1在NSCLC中表达水平升高,可能通过激活PI3K/AKT通路影响NSCLC细胞的增殖、迁移、侵袭,促进NSCLC的发生发展。Objective:To investigate the biological function and possible mechanism of long non-coding RNA(lncRNA)MNX1-AS1(lncRNA MNX1-AS1)in non-small cell lung cancer(NSCLC).Methods:The cancer tissue and adjacent tissue samples of 75 NSCLC patients who underwent surgical treatment in our hospital from June 2017 to March 2019 were taken.RT-qPCR method was performed to detect the expression level of lncRNA MNX1-AS1 in the samples,the correlation between the expression of lncRNA MNX1-AS1 and the clinicopathological characteristics and prognosis of NSCLC was analyzed;the NSCLC cell line PC-9 cells were cultured in vitro and transfected with si-MNX1-AS1(si-MNX1-AS1 group)and si-NC(si-NC group),respectively.the proliferation of PC-9 cells in each group was detected by MTT assay and EdU assay;Transwell chamber assay was performed to detect cell migration and invasion;Western blot assay was performed to detect the expression of PI3K/AKT pathway-related proteins.Results:The expression level of lncRNA MNX1-AS1 was obviously higher in NSCLC cancer tissues and cell lines(P<0.05).The expression of lncRNA MNX1-AS1 was associated with TNM stage and lymphatic metastasis(P<0.05),Kaplan-Meier survival analysis showed that the 3-year cumulative survival rate of patients in the high expression group of lncRNA MNX1-AS1 was obviously lower than that in the low expression group(42.11%vs 81.08%,χ^(2)=11.132,P=0.001);compared with the si-NC group,the proliferation,migration and invasion abilities of PC-9 cells in the si-MNX1-AS1 group and the protein expression levels of p-PI3K,p-AKT and p-mTOR were obviously decreased(P<0.05).Conclusion:The expression level of lncRNA MNX1-AS1 is increased in NSCLC,which may affect the proliferation,migration and invasion of NSCLC cells by activating the PI3K/AKT pathway,and promote the occurrence and development of NSCLC.

关 键 词：非小细胞肺癌 长链非编码RNA MNX1-AS1 PI3K/AKT通路 迁移 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]