小麦籽粒中脱氧雪腐镰刀菌烯醇(DON)积累及其与容重的相关性分析  

Analysis of deoxynivalenol (DON) accumulation in wheat kernels and its correlation with test weight

作　　者：何贤芳[1] 董旭 赵莉[1] 都斌斌 卢茂昂 邓骋 汪建来[1] HE Xianfang;DONG Xu;ZHAO Li;DU Binbin;LU Mao′ang;DENG Cheng;WANG Jianlai(Crop Institute,Anhui Academy of Agricultural Sciences,Hefei 230031,China;Anhui Institute of Plant Protection and Agro-products Safety,Hefei 230031,China;Faculty of Biological and Pharmaceutical Engineering,West Anhui University,Lu′an 237012,China;College of Agronomy,Anhui Agricultural University,Hefei 230036,China)

机构地区：[1]安徽省农业科学院作物研究所,安徽合肥230031 [2]安徽省植物保护与农产品质量安全研究所,安徽合肥230031 [3]皖西学院生物与制药工程学院,安徽六安237012 [4]安徽农业大学农学院,安徽合肥230036

出　　处：《北方农业学报》2022年第5期1-9,共9页Journal of Northern Agriculture

基　　金：沿淮地区麦稻周年绿色高效均衡增产技术研究与示范项目;安徽省小麦良种联合攻关(2021-);安徽省现代农业产业技术体系建设专项资金(340000222426000100009)。

摘　　要：【目的】探明自然发病条件下不同小麦品种籽粒中脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)积累量及其对籽粒赤霉病病粒率(FDK)和容重的影响,为小麦抗赤霉病品种选育提供参考。【方法】利用液相色谱串联质谱法(LC-MS/MS)测定65份不同来源小麦品种籽粒中DON、3A-DON(3-acetyldeoxynivalenol)、15A-DON(15-acetyldeoxynivalenol)含量,分析不同DON含量下籽粒赤霉病病粒率(FDK)和容重(TW)的差异。【结果】65份小麦品种籽粒的FDK为0.20%~7.80%、DON含量为0.11~4.26 mg/kg、容重为703.00~791.70 g/L。FDK与DON、3A-DON、15A-DON和DON-Total含量呈极显著正相关(P<0.01),相关系数分别为0.514、0.549、0.529和0.521;与容重呈显著负相关(P<0.05),相关系数为-0.254。籽粒中DON、3A-DON、15A-DON与DON-Total含量呈极显著正相关(P<0.01),相关系数分别为0.999、0.911、0.899;与容重呈极显著负相关(P<0.01),相关系数分别为-0.502、-0.504、-0.481。从不同品种来源看,籽粒中DON积累量表现为江苏(1.51 mg/kg)<安徽(2.06 mg/kg)<河南(2.75 mg/kg)<山东(2.98 mg/kg);3A-DON含量表现为江苏(0.047 mg/kg)<安徽(0.075 mg/kg)<山东(0.111 mg/kg)<河南(0.113 mg/kg);15A-DON表现为江苏(0.059 mg/kg)<安徽(0.090 mg/kg)<山东(0.132 mg/kg)<河南(0.141 mg/kg);DON-Total表现为江苏(1.61 mg/kg)<安徽(2.23 mg/kg)<河南(3.00 mg/kg)<山东(3.23 mg/kg)。小麦籽粒中DON≤2 mg/kg的品种数及占比表现为安徽(18个,64.29%)>江苏(13个,61.90%)>山东(2个,22.22%)>河南(1个,14.29%);容重均值表现为江苏(758.57 g/L)>安徽(751.90 g/L)>河南(739.71 g/L)>山东(735.27 g/L)。从不同品种特性看,筛选出8个DON≤2 mg/kg且容重≥770.00 g/L的小麦品种,其中,半冬性品种7个,分别为淮麦40、淮麦44、农麦152、荃麦725、涡麦9号、徐农029、淮麦45;春性品种1个,为国红6号。【结论】65份小麦品种籽粒FDK、DON含量和容重差异较大;随着FDK的增大,籽粒中3A-DON、15A-DON及DON-Total的积累量逐渐增加【Objective】To explore the accumulation of deoxynivalenol(DON)in the kernels of different wheat varieties under natural disease conditions and the effect on Fuasrium diseased kernel rate(FDK)and test weight,so as to provide reference for breeding wheat varieties resistant to Fuasrium.【Methods】The content of DON,3A-DON(3-acetyldeoxynivalenol)and 15A-DON(15-acetyldeoxynivalenol)in the kernels of 65 wheat varieties from different sources were tested by liquid chromatography-tandem mass spectrometry(LC-MS/MS)to analyze the difference in Fuasrium diseased kernel rate(FDK)and test weight(TW)under different DON content.【Results】The FDK of the 65 wheat varieties ranged from 0.20%to 7.80%.The content of DON was 0.11 to 4.26 mg/kg.The test weight was 703.00 to 791.70 g/L.With correlation coefficients of 0.514,0.549,0.529 and 0.521,respectively,FDK was significantly positively correlated with the contents of DON,3A-DON,15A-DON and DON-Total(P<0.01),and was significantly negatively correlated with test weight(P<0.05),with correlation coefficients of-0.254.With correlation coefficients of 0.999,0.911 and 0.899,respectively,the kernel contents of DON,3A-DON,15A-DON had significant positive correlation(P<0.01)with DON-Total,and significant negative correlation with test weight(P<0.01),with correlation coefficients of-0.502,-0.504 and-0.481,respectively.In terms of the source of the varieties,the kernel accumulation of DON was lowest in Jiangsu(1.51 mg/kg),followed by Anhui(2.06 mg/kg),Henan(2.75 mg/kg)and Shandong(2.98 mg/kg).The content of 3A-DON was lowest in Jiangsu(0.047 mg/kg),followed by Anhui(0.075 mg/kg),Shandong(0.111 mg/kg)and Henan(0.113 mg/kg).The content of 15A-DON was lowest in Jiangsu(0.059 mg/kg),followed by Anhui(0.090 mg/kg),Shandong(0.132 mg/kg)and Henan(0.141 mg/kg).The content of DON-Total was lowest in Jiangsu(1.61 mg/kg),followed by Anhui(2.23 mg/kg),Henan(3.00 mg/kg)and Shandong(3.23 mg/kg).The number and proportion of varieties with DON no more than 2 mg/kg in wheat kernels were as follows:An

关 键 词：小麦 脱氧雪腐镰刀菌烯醇 赤霉病病粒率 容重 液相色谱串联质谱法 

分 类 号：S432.1[农业科学—植物病理学]