生物样本速冻装置导冷平板性能模拟  

Simulation on Performance of Cooling Capacity Derivation Plate of Quick-freezing Device for Biological Samples

作　　者：方舒超 陈州旗 刘宝林[1] FANG Shuchao;CHEN Zhouqi;LIU Baolin(Institute of Biothermal Science,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学生物系统热科学研究所,上海200093

出　　处：《制冷技术》2022年第5期18-22,共5页Chinese Journal of Refrigeration Technology

基　　金：国家自然科学基金(No.51776130);国家重点研发计划(No.2017YFD0400404)。

摘　　要：为了研究冻存装置的部件结构对生物样本冷冻复苏后的成活率影响,本文设计了以80 W斯特林制冷机为冷源的新型样本速冻装置,对导冷平板及冷台等影响冷量传递效率的关键部件进行了材料选择,并基于COMSOL Multiphysics有限元模拟软件对导冷平板结构进行了模拟分析和实验验证。结果表明:对于中心管样本,采用2 mm导冷平板时降温最快,可达0.27 K/s,300 s末终温最低可达212 K,从降温初始阶段就与其他三组逐渐拉开差距;对于边界管样本,4种形状的导冷平板300 s末终温偏差在5.5%以内。To study the influence of the structure of the cryopreservation device on the survival rate of biological samples after freezing and thawing. In this study, a new sample quick-freezing platform using an 80 W Stirling refrigerator as a cold source is designed, and selected the materials for the key components affecting cooling capacity such as the cooling capacity derivation plate and the cooling stage. The simulation analysis of the cooling capacity derivation plate structure is based on COMSOL Multiphysics finite element simulation software. The results show that: for the central tube samples, when using the 2 mm cooling capacity derivation plate, the fastest cooling rate can be 0.27 K/s when using a 2 mm conductive cooling plate, and the lowest final temperature can be as low as 212 K at the end of 300 s, and the gap with the other three groups is gradually opened from the initial stage of cooling. For boundary tube samples, the final temperature deviation at the end of 300s of the four shapes of the cooling capacity derivation plate is less than 5.5%.

关 键 词：低温保存 速冻装置 导冷平板 数值模拟 

分 类 号：TQ051.5[化学工程] R318.52[医药卫生—生物医学工程]