Wnt/β-catenin信号通路探究羌活鱼超微粉抗去卵巢大鼠骨质疏松的作用机制  

Based on Wnt/β-Catenin signaling pathway to explore the effect and mechanism of Bing’s giant salamander ultrafine comminution on osteoporosis in ovariectomized rats

作　　者：钟建春 谢兴文 李鼎鹏 姜朝阳 杨东东 郭云龙 刘俊鹏 ZHONG Jianchun;XIE Xingwen;LI Dingpeng;JIANG Zhaoyang;YANG Dongdong;GUO Yunlong;LIU Junpeng(Gansu Traditional Chinese Medicine Hospital,Lanzhou 730050,China;The Second People’s Hospital of Gansu Province,Lanzhou 730030,China;Affiliated Hospital of Northwest University for Nationalities,Lanzhou 730030,China;Gansu University of traditional Chinese Medicine,Lanzhou 730030,China)

机构地区：[1]甘肃省中医院,甘肃兰州730050 [2]甘肃省第二人民医院,甘肃兰州730030 [3]西北民族大学附属医院,甘肃兰州730030 [4]甘肃中医药大学,甘肃兰州730030

出　　处：《中国骨质疏松杂志》2022年第12期1750-1754,1764,共6页Chinese Journal of Osteoporosis

摘　　要：目的探究羌活鱼超微粉对去卵巢大鼠骨代谢、骨微观结构及Wnt/β-catenin信号通路的影响。方法选取75只4月龄SPF级雌性SD大鼠,随机分为假手术组、模型组、羌活鱼超微粉组、羌活鱼普通粉组、仙灵骨葆组,除假手术组外均采用去势法建立骨质疏松症动物模型。药物干预12周后通过ELISA检测各组血清骨代谢指标、通过Micro-CT扫描测定各组大鼠的股骨骨微结构变化、运用Western Blot法测定骨组织中Wnt/β-catenin信号通路相关蛋白的表达。结果药物干预12周后,与模型组相比,羌活鱼组、仙灵骨葆组的ALP、OPG、PINP及BMD、BV/TV、Tb.Th、Tb.N显著升高,SMI、Tb.Sp、TRACP显著降低,Wnt2、β-catenin、Runx2的蛋白表达增加,但仍较假手术组低,差异有统计学意义(P<0.05);羌活鱼超微粉组在改善大鼠骨代谢、骨微观结构及蛋白表达方面均优于羌活鱼普通粉组,差异具有统计学意义(P<0.05)。结论羌活鱼能够延缓去卵巢大鼠骨质疏松的发生、发展,且超微粉剂优于普通粉剂,其作用机制可能与激活Wnt/β-catenin信号通路有关。Objective To investigate the effects of Bing’s giant salamander ultra micro comminution on bone metabolism,bone microstructure andWnt/β-catenin signaling pathway in ovariectomized rats.Methods 75 SPF female SD rats aged 4-month-old were randomly divided into 5 groups:shamoperation group,model group,ultra micro comminution group of Bing’s giant salamander.Osteoporosis,ordinary comminution group of Bing’s giant salamander,Osteoporosisand Xianlinggubao group.Except the sham operation group,the animal model of osteoporosis was established by castration.After 12 weeks of drug intervention,serum bone metabolism indexes were detected by ELISA,femoral bone microstructure changes were measured by micro CT scanning,and the expression of Wnt/β-catenin signaling pathway related proteins in bone tissue was measured by Western blot.Results After 12 weeks of drug intervention,compared with the model group,ALP,OPG,PINP,BMD,BV/TV and in Notopterygii group and Xianlinggubao group TB.Th,Tb.N increased significantly,SMI and TB.SP and TRACP decreased significantly,Wnt/β-Catenin The protein expression of catenin and Runx2 increased,but it was still lowerthan that in sham operation group(P<0.05);The ultra micro comminution group was superior to the ordinary comminution group in improving bone metabolism,bone microstructure and protein expression inrats(P<0.05).Conclusion Bing’s giant salamander can delay the occurrence and development of osteoporosis in ovariectomized rats,and ultra-fine powder is better than ordinary powder,and its mechanism may be related to the activation of Wnt/β-Catenin signaling pathway.

关 键 词：羌活鱼 骨质疏松症 MICRO-CT 超微粉碎 WNT/Β-CATENIN信号通路 

分 类 号：R274.9[医药卫生—中西医结合]