硫化物胁迫对缢蛏血液SO_(4)^(2-)浓度及 SULT1B1-12 基因表达的影响  

Effects of sulfide stress on blood SO_(4)^(2-) concentration and SULT1B1-12 gene expression in Sinonovacula constricta

作　　者：孙慧妙 沈伟良 陈彩芳 林志华 韩庆喜[2] Sun Huimiao;Shen Weiliang;Chen Caifang;Lin Zhihua;Han Qingxi(Zhejiang Key Laboratory of Aquatic Germplasm Resources,College of Biological and Environmental Sciences,Zhejiang Wanli University,Ningbo 315100,China;School of Marine Sciences,Ningbo University,Ningbo 315823,China;Ningbo Academy of Oceanology and Fishery,Ningbo 315012,China;Ninghai Institute of Mariculture Breeding and Seed Industry,Zhejiang Wanli University,Ninghai 315604,China)

机构地区：[1]浙江万里学院生物与环境学院浙江省水产种质资源高效利用技术研究重点实验室,浙江宁波315100 [2]宁波大学海洋学院,浙江宁波315823 [3]宁波市海洋与渔业研究院,浙江宁波315012 [4]浙江万里学院宁海海洋生物种业研究院,浙江宁海315604

出　　处：《海洋学报》2023年第1期62-70,共9页

基　　金：浙江省公益技术应用研究项目(LGN22C190025);国家重点研发计划“蓝色粮仓”科技创新课题(2018YFD0901405,2020YFD0900802);财政部和农业农村部:国家现代农业产业技术体系项目(CARS-49);浙江省科技重点研发计划(2019C02054,2021C02069-7)。

摘　　要：作为典型的埋栖型滩涂贝类,缢蛏(Sinonovacula constricta)常暴露在富含硫化物的环境中,并表现出较强的硫化物耐受能力。胞质磺基转移酶1B1(SULT1B1)位于硫代谢途径下游,是催化磺化反应的关键酶,在甲状腺激素(THs)等内源性物质的生物转化过程中发挥重要作用。为研究ScSULT1B1-12基因在缢蛏耐硫中的作用,本研究采用生物信息学方法分析了其序列特征,并结合血液中SO_(4)^(2-)浓度变化,开展其组织表达及不同浓度(50μmol/L、150μmol/L、300μmol/L)硫化物胁迫72 h后的表达特征研究。结果表明,ScSULT1B1-12基因全长cDNA为1100 bp,含有897 bp的开放阅读框,编码298个氨基酸。序列分析表明,ScSULT1B1-12含有4个催化活性位点(_(56)K、_(104)N、_(106)H和_(134)A)、1个N端的PAPS结合域(YPKSGTXW)、1个C端的PAPS结合和二聚化域(RKGXXGDWKNXFTVXXE),表明其在结构上具有催化磺化反应的能力。组织分布表明,ScSULT1B1-12基因在鳃中高表达,其次为闭壳肌和肝胰腺。硫化物胁迫后缢蛏血液中SO_(4)^(2-)浓度呈下降趋势,ScSULT1B1-12基因的表达模式也在波动中呈下降趋势,表明硫酸盐可进一步被活化生成磺化反应的供体,而ScSULT1B1-12介导的磺化反应受抑制后可使缢蛏体内THs保持在一定水平,以加强其代谢机能和免疫功能,使机体适应高硫化物的不良环境。As a typical dwelled tidal shellfish,Sinonovacula constricta is often exposed to sulfide-rich environment and shows strong sulfide tolerance.The cytosolic sulfotransferase 1B1(SULT1B1)is located at downstream of the sulfur metabolism pathway,while it is a key enzyme catalyzing the sulfation reaction and plays an important role in the biotransformation of endogenous substances such as thyroid hormones(THs).In order to study the role of ScSULT1B1-12 in sulfur resistance,the sequence characteristics were analyzed by bioinformatics method.Combined with the changes of blood SO_(4)^(2-)concentration,the spatial expression and temporal expression profiles during 72 h sulfide stress(50μmol/L,150μmol/L,300μmol/L)were studied.The full-length cDNA of ScSULT1B1-12 gene was 1100 bp,containing an open reading frame of 897 bp,and encoding 298 amino acids.Sequence analysis showed that ScSULT1B1-12 contains four catalytic active sites(_(56)K,104N,_(106)H,and _(134)A),one PAPS binding domain(YPKSGTXW)at N terminal,and one PAPS binding and dimerization domain(RKGXXGDWKNXFTVXXE)at C terminal,indicating that it was structurally able to catalyze the sulfation reaction.Spatial expression showed that ScSULT1B1-12 was highly expressed in gills,followed by the adductor muscle and hepatopancreas.Blood SO_(4)^(2-)concentration decreased,and the expression patterns of ScSULT1B1-12 also declined with fluctuation after sulfide stress,indicating that sulfate can be further transformed to sulfated donors,and ScSULT1B1-12-mediated sulfation may be inhibited to keep THs at a certain level in S.constricta,in order to strengthen the metabolic and immune functions,and make the organism adapt the adverse environment of high sulfide.

关 键 词：缢蛏 ScSULT1B1-12 硫化物胁迫 时间表达 硫酸根 

分 类 号：Q955[生物学—动物学] Q786