ADAMTS7在人胃癌中的表达及临床意义  

Expression and clinical significance of ADAMTS 7 in human gastric cancer

作　　者：汪虎[1] 李靖[1] 谢波[1] 张晨嵩 潘成武[1] 张新鑫 王辉 贾建光[1] WANG Hu;LI Jing;XIE Bo;ZHANG Chensong;PAN Chengwu;ZHANG Xinxin;WANG Hui;JIA Jianguang(Department of Oncology Surgery,The First Affiliated Hospital of Bengbu Medical College,Anhui 233000,China)

机构地区：[1]蚌埠医学院第一附属医院肿瘤外科,安徽蚌埠233000

出　　处：《淮海医药》2023年第1期1-5,10,共6页Journal of Huaihai Medicine

基　　金：蚌埠医学院自然科学类项目(BYKY2019099ZD);安徽省教育厅2021年度高校自然科学重点项目(KJ2021A0795);蚌埠医学院“512人才培育计划”(by51202207)。

摘　　要：目的:利用生物信息学方法分析含Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶7(ADAMTS7)在人胃癌中的表达及临床意义,并进一步预测其可能的作用机制。方法:从癌症基因组图谱(TCGA)数据库下载ADAMTS7基因表达数据及临床数据,应用R软件对数据整理比较其在不同肿瘤组织中的表达情况;对差异基因批量化生存分析、独立预后分析及绘制列线图;分析ADAMTS7基因表达与胃癌不同临床病理特征的相关性及对患者预后的影响;应用基因集富集分析(GSEA)对目的基因行富集分析,预测目的基因调控胃癌进展及预后可能的机制。结果:ADAMTS7在不同种类肿瘤中表达不同,其表达水平一般上调。其中,ADAMTS7在胃癌组织中显著高表达。生存分析表明,ADAMTS7高表达患者具有较差的总生存率及较差的疾病特异性生存率。多因素Cox回归分析显示ADAMTS7高表达、N3期、年龄大于65岁及残余肿瘤是胃癌患者预后较差的因素。基因集富集分析结果表明MAPK信号通路和Hedgehog信号通路可能是ADAMTS7高表达影响胃癌的机制。结论:ADAMTS7高表达水平在胃癌发生发展过程中可能起着重要作用,有望为胃癌诊治和预后判断提供新的靶点。Objective:To analyze the expression and clinical significance of disintegrin and metalloproteinase with thrombospondin motifs 7(ADAMTS7)in human gastric cancer using bioinformatics methods,and to further predict its possible mechanism of action.Methods:The ADAMTS7 gene expression data and clinical data were downloaded from the Cancer Genome Atlas(TCGA)database,and R software was used to organize the data to compare its expression in different tumor tissues;batch survival,independent prognosis and nomogram were performed for the differential genes;the ADAMTS7 gene expression was analyzed.The correlation of the expression with different clinicopathological features of gastric cancer and its impact on prognosis of patients were analyzed;gene set enrichment analysis(GSEA)was used to perform enrichment analysis on the target gene to predict the possible mechanism of target gene regulating the progression and prognosis of gastric cancer.Results:ADAMTS7 was expressed differently in different types of tumors,and its expression level was generally up-regulated.Among them,ADAMTS7 was significantly overexpressed in gastric cancer tissues.Survival analysis showed that patients with high ADAMTS7 expression had poorer overall survival and poorer disease-specific survival.Multivariate Cox regression analysis showed that ADAMTS7 high expression,N3 stage,age over 65 years and residual tumor were prognostic factors for poor survival of gastric cancer.The results of gene set enrichment analysis indicated that MAPK signaling pathway and Hedgehog signaling pathway may be the mechanism by which high ADAMTS7 expression affects gastric cancer.Conclusion:High expression level of ADAMTS7 may play an important role in the occurrence and development of gastric cancer,and is expected to become a new target for the diagnosis,treatment and prognosis of gastric cancer.

关 键 词：胃肿瘤 ADAMTS7 生物信息学 进展及预后 

分 类 号：R735.2[医药卫生—肿瘤]