右美托咪定对大鼠蛛网膜下腔出血后早期脑损伤的影响  

Effect of Dexmedetomidine on Early Brain Injury After Subarachnoid Hemorrhage in Rats

作　　者：陈湘姣 杨豪 梁辉[1] CHEN Xiang-jiao;YANG Hao;LIANG Hui(Department of Neurology,the First Affiliated Hospital of Hunan Normal University/Hunan Provincial People's Hospital,Changsha 410000,Hunan,China)

机构地区：[1]湖南师范大学附属第一医院/湖南省人民医院神经内科,湖南长沙410000

出　　处：《医学信息》2022年第23期61-68,共8页Journal of Medical Information

摘　　要：目的探讨右美托咪定(DEX)干预对蛛网膜下腔出血(SAH)后自噬活性的影响以及其在SAH后早期脑损伤中的作用。方法将48只SD大鼠随机分为4组:Sham组、SAH组、SAH+DEX组、SAH+3-MA组,每组12只,各组再分为两个亚组,每组6只。比较各组神经功能、脑含水量、伊文思蓝含量、细胞凋亡、Beclin-1和LC3-Ⅱ蛋白表达水平及自噬体数目和形态。结果①SAH组、SAH+DEX组、SAH+3-MA组造模后24、72 h神经功能评分均低于Sham组,SAH+DEX组、SAH+3-MA组造模后24 h神经功能评分高于SAH组(P<0.05),但SAH+DEX组与SAH+3-MA组造模后24、72 h神经功能评分比较,差异无统计学意义(P>0.05);②SAH组、SAH+DEX组、SAH+3-MA组大鼠造模后24、72 h脑含水量、脑组织EB含量均高于Sham组,SAH组造模后24、72 h脑组织含水量高于SAH+DEX组和SAH+3-MA组(P<0.05),但SAH+DEX组与SAH+3-MA组造模后24、72 h脑含水量、脑组织EB含量比较,差异无统计学意义(P>0.05);③SAH组、SAH+DEX组、SAH+3-MA组造模后24 h脑细胞凋亡率高于Sham组,SAH组造模后24 h脑细胞凋亡率高于SAH+DEX组、SAH+3-MA组(P<0.05);各组造模后72 h脑细胞凋亡率比较,差异无统计学意义(P>0.05);④透射电子显微镜下可见到大小不等的自噬空泡及自噬小体,其中SAH+DEX组、SAH+3-MA组自噬体数目较SAH组减少,而SAH+DEX组与SAH+3-MA组大鼠脑组织中自噬体数量无明显差异;⑤SAH组、SAH+DEX组、SAH+3-MA组造模后24、72 h Beclin-1蛋白表达量高于Sham组(P<0.05);SAH组造模后24、72 h Beclin-1和LC3-Ⅱ蛋白表达量均高于SAH+DEX组及SAH+3-MA组(P<0.05);SAH组、SAH+3-MA组造模后24、72 h LC3-Ⅱ蛋白表达量高于Sham组(P<0.05),但SAH+DEX组和Sham组LC3-Ⅱ蛋白表达量比较,差异无统计学意义(P>0.05)。结论DEX可通过抑制细胞自噬及细胞凋亡、保护血脑屏障在SAH后早期脑损伤中起神经保护作用。Objective To investigate the effect of dexmedetomidine(DEX)intervention on autophagy activity after subarachnoid hemorrhage(SAH)and its role in early brain injury after SAH.Methods Forty-eight SD rats were randomly divided into 4 groups:Sham group,SAH group,SAH+DEX group,SAH+3-MA group,with 12 rats in each group,and each group was divided into two subgroups,with 6 rats in each subgroups.The neurological function,brain water content,Evans blue content,cell apoptosis,Beclin-1 and LC3-II protein expression,autophagosome number and morphology were compared among the groups.Results①The neurological function scores of SAH group,SAH+DEX group and SAH+3-MA group were lower than those of Sham group at 24 and 72 h after modeling,and the neurological function scores of SAH+DEX group and SAH+3-MA group were higher than those of SAH group at 24 h after modeling(P<0.05),but there was no significant difference in neurological function score between SAH+DEX group and SAH+3-MA group at 24 and 72 h after modeling(P>0.05);②The brain water content and EB content in SAH group,SAH+DEX group and SAH+3-MA group were higher than those in Sham group at 24 and 72 h after modeling,and the brain water content in SAH group was higher than that in SAH+DEX group and SAH+3-MA group at 24 and 72 h after modeling(P<0.05),but there was no significant difference in brain water content and EB content between SAH+DEX group and SAH+3-MA group at 24 and 72 h after modeling(P>0.05).The apoptosis rate of brain cells in SAH group,SAH+DEX group and SAH+3-MA group was higher than that in Sham group(P<0.05);③The apoptosis rate of brain cells in SAH group,SAH+DEX group and SAH+3-MA group was higher than that in Sham group at 24 h after modeling,and the apoptosis rate of brain cells in SAH group was higher than that in SAH+DEX group and SAH+3-MA group at 24 h after modeling(P<0.05),but there was no significant difference in the apoptosis rate of brain cells at 72 h after modeling in each group(P>0.05);④Autophagic vacuoles and autophagosomes with differe

关 键 词：蛛网膜下腔出血 早期脑损伤 右美托咪定 自噬 血脑屏障 凋亡 

分 类 号：R743.35[医药卫生—神经病学与精神病学]